To solve the problem of springback in traditional multi-point forming (MPF), a new process, namely, with individually controlled force–displacement (MPF-ICFD), is proposed. In this manuscript, MPF and MPF-ICFD are compared. Taking cylindrical surface as research object, change plate analysed by experiments, distribution strain wall thickness numerical simulation. Theoretical analysis used to ca...

PURPOSE To establish monoclonal antibodies (mAb) against megakaryocyte potentiation factor (MPF) and detect MPF in the blood of patients with mesothelioma. EXPERIMENTAL DESIGN Mice were immunized with a purified recombinant human MPF-rabbit-Fc fusion protein and with MPF. Several hybridomas producing mAbs to MPF were established. A double-determinant (sandwich) ELISA was constructed using mAb...

We have examined the regulation of maturation-promoting factor (MPF) activity in the mitotic and meiotic cell cycles of Xenopus laevis eggs and oocytes. To this end, we developed a method for the small scale extraction of eggs and oocytes and measured MPF activity in extracts by a dilution end point assay. We find that in oocytes, MPF activity appears before germinal vesicle breakdown and then ...

Although maturation/M phase promoting factor (MPF) can activate autonomously in Xenopus egg cytoplasm, indirect evidence suggests that nuclei and centrosomes may focus activation within the cell. We have dissected the contribution of these structures to MPF activation in fertilized eggs and in egg fragments containing different combinations of nuclei, centrosomes, and microtubules by following ...

Figure 1: In step 1, cyclin is synthesized de novo. Newly synthesized cyclin may be unstable (step 2). Cyclin combines with cdc2-P (step 3) to form pre-maturation promoting factor (preMPF). At some point after heterodimer formation, the cyclin subunit is phosphorylated. ... The cdc2 subunit is then dephosphorylated (step 4) to form active MPF. In principle, the activation of MPF may be opposed ...

During summer, melt ponds have a significant influence on Arctic sea-ice albedo. The melt pond fraction (MPF) also has the ability to forecast the Arctic sea-ice in a certain period. It is important to retrieve accurate melt pond fraction (MPF) from satellite data for Arctic research. This paper proposes a satellite MPF retrieval model based on the multi-layer neural network, named MPF-NN. Our ...

M-phase Promoting Factor (MPF; the cyclin B-cdk 1 complex) is activated at M-phase onset by removal of inhibitory phosphorylation of cdk1 at thr-14 and tyr-15. At M-phase exit, MPF is destroyed by ubiquitin-dependent cyclin proteolysis. Thus, control of MPF activity via inhibitory phosphorylation is believed to be particularly crucial in regulating transition into, rather than out of, M-phase. ...

It has been known for some time that maturation-promoting factor (MPF) appears in a wide variety of eukaryotic cells at M phase and exerts equal M-phase-promoting activity in both meiotic cells and mitotic cells in a non-specific manner. MPF was extracted from cdc20 mutant cells of the yeast Saccharomyces cerevisiae synchronized at M phase by incubation at the restrictive temperature. When inje...

IncP-type plasmids are broad-host-range conjugative plasmids. DNA translocation requires DNA transfer-replication functions and additional factors required for mating pair formation (Mpf). The Mpf system is located in the cell membranes and is responsible for DNA transport from the donor to the recipient. The Mpf complex acts as a receptor for IncP-specific phages such as PRD1. In this investig...

Back extensor muscle fatigability and its relationship to body mass index (BMI) was measured in 12 chronic nonspecific low back pain (CNLBP) patients (7 women and 5 men) and 12 healthy age-and gender-matched controls. Subjects performed Sørensen back isometric endurance test until exhaustion while EMG spectral mean power frequency (MPF) over the lumbar erector spinae muscle and endurance time w...