Increased activation of ERK signaling has been reported in breast cancer models of acquired tamoxifen resistance. Here, we examined the expression of Mitogen-Activated Protein Kinase Phosphatases (MKPs) 1 and 2 following tamoxifen treatment and the effects of MKP-1/MKP-2 overexpression on tamoxifen sensitivity. Treatment of MCF7 breast cancer cells with tamoxifen increased MKP-2, but not MKP-1,...

atherogenesis. Methods and Results: Mice with homozygous deficiency in MKP-1 (MKP-1 / ) were bred with apolipoprotein (Apo)E-deficient mice (ApoE / ) and the 3 MKP-1 genotypes (MKP-1 / /ApoE / ; MKP-1 / /ApoE / and MKP-1 / /ApoE / ) were maintained on a normal chow diet for 16 weeks. The 3 groups of mice exhibited similar body weight and serum lipid profiles; however, both MKP-1 / and MKP-1 / m...

The multidimensional knapsack problem (MKP) is a classic in combinatorial optimisation. Several authors have proposed to use surrogate relaxation compute upper bounds for the MKP. In their papers, dual solved heuristically. We show that, using modern simplex solver as subroutine, one can solve exactly reasonable computing times. On other hand, resulting bound tends be strong only relatively sma...

RATIONALE Multiple protein kinases have been implicated in cardiovascular disease; however, little is known about the role of their counterparts: the protein phosphatases. OBJECTIVE To test the hypothesis that mitogen-activated protein kinase phosphatase (MKP)-1 is actively involved in atherogenesis. METHODS AND RESULTS Mice with homozygous deficiency in MKP-1 (MKP-1(-/-)) were bred with ap...

BACKGROUND MAP kinase phosphatase 3 (MKP-3) is known to attenuate the ERK signaling pathway. It has been recently demonstrated that MKP-3 is also a player in promoting hepatic glucose output in obese state by interacting and activating FOXO1. Reduction of hepatic MKP-3 expression is sufficient to reduce blood glucose levels in both diet-induced and genetically obese mice. METHODOLOGY/PRINCIPA...

This paper presents an efficient experimental-numerical analysis of fracture mechanics in magnesium phosphate cement (MPC) based on the structural and mechanical properties its constituents including potassium hexahydrate (MKP), oxide (MgO) pores. At micro-scale, energy material strength solid phases were obtained relying combination nanoindentation experiments simulation. The X-ray computed to...

Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP kinase cascades in a variety of cellular processes, including proliferation and stress responsiveness. Although MKP-1 expression is induced by a broad array of extracellular stimuli, the mechanisms mediating its induction remain poorly understood. Here we ...

Mitogen-activated protein kinase (MAPK) phosphatase (MKP)-1 is a member of the MKP family that negatively regulates MAPK signaling. MKP-1 has been implicated in cell survival in response to stressful stimuli, including anticancer treatment, but its role in cisplatin resistance is not fully understood. Here, we show that cisplatin induces MKP-1 in several human cancer cell lines. Induction of MK...

Mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) is the MAPK phosphatase family member that negatively regulates MAPK signaling. Our previous study showed that MKP-1 is involved in cisplatin resistance, but the mechanism underlying its resistance is not understood. Here, we show that ERK2-mediated MKP-1 expression is critical for cisplatin resistance. Specifically, we showed that i...

We have previously reported that MAPK phosphatase-1 (MKP-1/CL100) is a thrombin-responsive gene in endothelial cells (ECs). We now show that VEGF is another efficacious activator of MKP-1 expression in human umbilical vein ECs. VEGF-A and VEGF-E maximally induced MKP-1 expression in ECs; however, the other VEGF subtypes had no effect. Using specific neutralizing antibodies, we determined that V...