High aerobic glycolysis, as one of the hallmarks of cancer cells, requires nicotinamide adenine dinucleotide (NAD+) as a vital co-factor, to guarantee the flow of glycolysis. Malate dehydrogenase (MDH), as an important enzyme in cancer metabolism, is a source of NAD+ additional to lactate dehydrogenase (LDH). The current study aimed to elucidate the kinetic parameters of MDH in human breast can...

Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone fractionation of soluble plasmodial homogenates acidified to pH 5.0. The M-MDH...

Malate dehydrogenases (MDHs) play crucial roles in the physiological processes of plant growth and development. In this study, 13 and 25 MDH genes were identified from Gossypium raimondii and Gossypium hirsutum, respectively. Using these and 13 previously reported Gossypium arboretum MDH genes, a comparative molecular analysis between identified MDH genes from G. raimondii, G. hirsutum, and G. ...

We present a new dynamic hashing scheme for disk-based databases, called Multi-Directory Hashing (MDH). MDH uses multiple hash directories to access a file. The size of each hash directory grows dynamically with the file size. The advantages of MDH are enhanced concurrency, improved bucket utilization and smaller total directory size than single-directory hashing. The expected utilization of MD...

In illuminated chloroplasts, one mechanism involved in reduction-oxidation (redox) homeostasis is the malate-oxaloacetate (OAA) shuttle. Excess electrons from photosynthetic electron transport in the form of nicotinamide adenine dinucleotide phosphate, reduced are used by NADP-dependent malate dehydrogenase (MDH) to reduce OAA to malate, thus regenerating the electron acceptor NADP. NADP-MDH is...

The genes (mdh) encoding malate dehydrogenase (MDH) from the mesophile Chlorobium vibrioforme and the moderate thermophile C. tepidum were cloned and sequenced, and the complete amino acid sequences were deduced. When the region upstream of mdh was analyzed, a sequence with high homology to an operon encoding ribosomal proteins from Escherichia coli was found. Each mdh gene consists of a 930-bp...

late: NAD oxidoreductase; EC 1.1.1.37) is an enzyme very commonly occurring in animals, plants and microorganisms. MDH catalyzes the oxidation (dehydration) of L-malate to oxaloacetate in the presence of NAD+ as a cofactor: This article is an overview of literature data on the structure, properties, functions and genetic control of the enzyme malate dehydrogenase (MDH) in plants. In most of the...

Let X be a compact connected Riemann surface of genus g ≥ 2, and let MDH be the rank one Deligne–Hitchin moduli space associated to X. It is known thatMDH is the twistor space for the hyper-Kähler structure on the moduli space of rank one holomorphic connections on X. We investigate the group Aut(MDH) of all holomorphic automorphisms of MDH. The connected component of Aut(MDH) containing the id...

Pyruvate,Pi dikinase (PPDK, EC 2.7.9.1) and NADP-malate dehydrogenase (MDH, EC 1.1.1.82) were activated in the light and inactivated following a dark treatment in mesophyll protoplasts of maize. DCMU (up to 33 micromolar), an inhibitor of noncyclic electron transport, inhibited activation of MDH much more strongly than it did PPDK. Antimycin A (6.6-33 micromolar), an inhibitor of cyclic photoph...

Malate dehydrogenase (MDH) is a key enzyme that catalyzes the reversible oxidation of malate to oxaloacetate and plays a crucial role in various cellular processes, such as cell expansion, wall thickening and cell elongation. Although individual genes belonging to MDH gene family have been partially identified in various plants, there have been no reports of a genome-wide characterization of th...