We describe here the characterization of five isolates of Mycobacterium simiae-like organisms representing a novel group based on whole-cell fatty acid analysis and genotypic evaluation. Two of the five isolates in this study, W55 and W58, were previously considered to belong to M. simiae serotype 2. Analysis of cellular fatty acids by gas-liquid chromatography indicated a close clustering of t...

Mycobacterium simiae is a multidrug-resistant, slowgrowing, nontuberculous mycobacterium (NTM) that has been rarely reported as a cause of pulmonary infections and disseminated disease in immunocompromised hosts, especially in patients with advanced AIDS [1]. Nontuberculous mycobacterium infections caused by M simiae have increasingly been reported as a cause for cervical lymphadenitis in immun...

Bacteremic pneumonia caused by Mycobacterium simiae in non-human immunodeficiency virus (HIV)-infected patients has rarely been reported. We describe a non-HIV-infected adult with refractory bacteremia, osteomyelitis, and colonization of M. simiae in the respiratory tract who subsequently developed fatal bacteremic pneumonia. The isolate was confirmed as M. simiae by 16S rRNA gene analysis.

Mycobacterium simiae is a non-tuberculosis mycobacterium causing pulmonary infections in both immunocompetent and imunocompromized patients. We announce the draft genome sequence of M. simiae DSM 44165(T). The 5,782,968-bp long genome with 65.15% GC content (one chromosome, no plasmid) contains 5,727 open reading frames (33% with unknown function and 11 ORFs sizing more than 5000 -bp), three rR...

conclusion the high discriminative power of hsp65 pattern particularly hpaii digestion, provide an exact and costeffective method for rapid identification of m. simiae strains among registered pulmonary cases. results of 962 culture positive specimens, 17(1.7%) were identified as m. simiae species; majority of them were multidrug-resistance (12; 70.5%). the overall detection rate by tb11, tb12 ...

Various species of nontuberculous mycobacteria are known to cause nosocomial pseudo-outbreaks, but there have been no detailed reports of nosocomial Mycobacterium simiae pseudo-outbreaks. From April 1997 through February 2001, we recovered 65 M. simiae isolates from 62 patients at a community teaching hospital in Houston, Texas. The organism was grown in various water samples obtained in the ho...

Mycobacterium simiae is a non-tuberculous mycobacterium (NTM) endemic to the southwestern United States, the Middle East and Cuba, and has been isolated from tap water, including municipal water [1]. Although NTM form an integral part of the environment, they have potential to cause clinical disease in immunocompromised hosts [2–4]. M. simiae most commonly causes pulmonary disease, however, bon...

INTRODUCTION Several species of non-tuberculosis mycobacteria (NTM) can affect humans and can cause either symptomatic or asymptomatic infection.  This study aimed to determine the clinical and radiological manifestation, as well as the treatment, of M. simiae in patients in Masih Daneshvari Hospital, a TB referral hospital in Iran. METHODOLOGY This retrospective study involved all patients p...

This is a report of the first South Korean case of a lung disease caused by Mycobacterium simiae. The patient was a previously healthy 52-year-old female. All serial isolates were identified as M. simiae by multi-locus sequencing analysis, based on hsp65, rpoB, 16S-23S rRNA internal transcribed spacer, and 16S rRNA fragments. A chest radiography revealed deterioration, and the follow-up sputum ...

We examined the mycolic acid composition of 133 strains belonging to MAIS complex (Mycobacterium avium-M. intracellulare-M. scrofulaceum) and MAIS intermediate strains and the related species M. asiaticum, M. malmoense, M. shimoidei, and M. simiae. The analysis revealed that about 10% of the strains identified as M. avium-M. intracellulare complex by conventional cultural and biochemical tests ...