The optimization of adsorption-based operations requires an accurate model to represent the separation process. In the present study, single-component adsorption data of L-lysine on its imprinted polymer were fitted to Freundlich, Langmuir-type and Jovanovic-type models. L-Lysine imprinted polymer (LLIP) was prepared by free radical solution polymerization. The best estimate of the parameters o...

AIMS To evaluate two of the recent methods of coating microtitre plates in the enzyme linked immunosorbent assay (ELISA) for detecting human antibodies against meningococcal capsular polysaccharides A and C with a view to validating a specific meningococcal antibody assay for routine clinical use. METHODS Two four-layer ELISA protocols were standardised: one method utilised meningococcal poly...

The enantiospecific desorption kinetics of R- and S-propylene oxide (PO) from a Cu(100) surface modified by enantiomerically pure D- or L-lysine have been studied using temperature programmed desorption. These experiments have used R- or S-PO as the chiral probe for study of enantiospecific adsorption on Cu(100) surfaces modified with D- or L-lysine. This chiral probe/modifier/Cu system manifes...

A combination of in situ and ex situ surface plasmon resonance (SPR) imaging experiments is used to characterize the differential electrostatic adsorption of proteins and synthetic polypeptides onto photopatterned monolayers at gold surfaces. The nonspecific electrostatic adsorption of proteins onto negatively charged self-assembled monolayers (SAMs) of 11-mercaptoundecanoic acid (MUA) is found...

Monolayers of the polypeptide poly(L-lysine) (PL) are used to control the specific adsorption of proteins onto gold surfaces. A PL monolayer modified with biotin is electrostatically adsorbed onto a vapor-deposited gold Eilm that has been coated with a self-assembled monolayer of the alkanethiol 1 1-mercaptoundecanoic acid (MUA). The immobilized biotin moieties act as specific adsorption sites ...

We previously reported the development of a cellulose fiber based blood filter device containing immobilized protamine (termed protamine filter) that could be used to control both heparin- and protamine-induced complications during extracorporeal therapy. To achieve enhanced heparin adsorption on the fibers, we examined the possibility of utilizing the poly-L-lysine based amplification method t...

Alginate-poly-L-lysine-alginate (APA) microcapsules continue to be the most widely studied device for the immuno-protection of transplanted therapeutic cells. Producing APA microcapsules having a reproducible and high level of biocompatibility requires an understanding of the mechanisms of the immune response towards the implants. Here, we investigate the adsorption of immunoglobulins (IgG, IgM...

The potential of spherical lignin beads as an adsorbent to take up Llysine from aqueous solution was investigated. The kinetic data were estimated by the pseudo-first-order and pseudo-second-order models. The mechanism of adsorption was also studied using the Boyd model and Webber’s intraparticle diffusion model. The equilibrium data were modeled by the Langmuir, Freundlich, and Dubinin-Radushk...

It remains a formidable challenge to construct high-performance haemoperfusion adsorbents with fast adsorption kinetic and high capacity for efficiently removing bilirubin from human blood. In this work, we report facile yet efficient strategy manufacture lysine-modified macroporous cellulose/carbon nanotube microspheres (LMCMs) by surfactant micelles swelling followed modification lysine. The ...