Methods Stem Br (Vital Nutrients lot # 2890; Middletown, CT), was tested for authenticity and purity. Female C57BL/6J mice, (Jackson Laboratory; Bar Harbor, ME) received one bolus, i.p. injection of Br (12mg/kg) in 0.5ml of physiological saline and plasma collected at baseline 3, 6, 12 and 24 hours. All procedures were approved by the Animal Care Committee at University of CT Health Center. Pos...

MOTIVATION Data reduction of liquid chromatography-mass spectrometry (LC-MS) spectra can be a challenge due to the inherent complexity of biological samples, noise and non-flat baseline. We present a new algorithm, LCMS-2D, for reliable data reduction of LC-MS proteomics data. RESULTS LCMS-2D can reliably reduce LC-MS spectra with multiple scans to a list of elution peaks, and subsequently to...

The aim of this study was the development and validation of a new method for carbohydrates analyses obtained from woody biomass, as a step of bioethanol production. In this study, we have quantified carbohydrates in wood by gas chromatography (GC) after derivatization. Quantification of carbohydrates was made by liquid-liquid extraction, oximation and silylation and, finally, analysis by gas ch...

Quantitative target analysis by liquid chromatography coupled to mass spectrometry (LCMS) is ubiquitous in environmental, metabolomic and toxicological studies. Targeted LCMS methods are capable of the simultaneous determination of literally hundreds of analytes. Although acquiring of instrumental data is very fast, data post-processing i.e. quantification can be time consuming step (and)or dep...

water contamination due to the wide variety of pesticides used in agriculture is a global environmental pollution problem. in order to reach at sub-î¼gl-1 levels of detection, an efficient extraction technique is required. a simple, fast and economical method, dispersive liquid-liquid micro extraction (dllme), followed by gas chromatography-mass spectrometry was assessed for determining endosul...

Mass spectrometry has long been used in clinical laboratories. The first important applications were the use of gas chromatography–mass spectrometry (GC-MS) for drug screening and the diagnosis of organic acid metabolic disorders. More recently liquid chromatography–mass spectrometry [LC-MS; liquid chromatography–tandem mass spectrometry (LC-MS/MS)] has been introduced successfully into clinica...

word count: 215 Introduction word count: 414 Results and Discussion word count: 1,010 Abreviations: CYP, Cytochrome P450; 13cisRA, 13-cis retinoic acid; LCMS, Liquid chromatography-mass spectrometry. This article has not been copyedited and formatted. The final version may differ from this version. DMD Fast Forward. Published on April 26, 2010 as DOI: 10.1124/dmd.109.030866 at A PE T Jornals on...

Background: Determination of cyclosporine A (CsA) and tacrolimus (Tac) in dried blood spots (DBS) could enable drug monitoring in transplanted patients without the necessity of having to take venous blood samples. Therefore, we have developed a method for quantitative determination of calcineurin inhibitors (CNI) by liquid-chromatography-tandem mass spectrometry (LCMS). Methods: In a study with...

A validated method for the quantitative determination of venlafaxine, O-desmethylvenlafaxine in human plasma by tandem liquid chromatographymass spectrometry (HPLC/MS/MS) has been proposed. Sample preparation was carried out liquid-phase extraction with support (SLE, from «supported extraction») using HyperSEP cartridges and washing 3-methylbutyl ether. The measure target analytes on a triple q...