Membrane solubilization induced by Triton X-100 (TX-100) was investigated. Different membrane compositions and phase states were studied along the detergent titration. Expected profiles obtained but new information is provided. The fluorescence of nitrobenzoxadiazole (NBD)-labeled lipids indicates that liquid-ordered (Lo)/liquid-disordered (Ld) coexistence barely unaffected at sub-solubilizing ...

The effect of Triton X-100, Na cholate and Tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of Salmonella typhimurium was studied. The detergents were used in various concentrations (1.6 to 64 mM) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. Results showed that no inne...

the effect of triton x-100, na cholate and tween 80 on the solubilization of integral membrane proteins in intact cells, spheroplasts and inner-membrane fragments of salmonella typhimurium was studied. the detergents were used in various concentrations (1.6 to 64 mm) and cytochromes b and d were used as marker to monitor the solubilization of membrane-bound proteins. results showed that no inne...

Solubilization of membrane proteins by amphiphilic detergents represents a crucial step in studies of membrane proteins in which proteins and lipids in natural membranes are dissociated giving rise to mixed clusters of proteins, lipids and detergents in the aqueous dispersion. Although solubilization is a popular method, physicochemical principles underlying solubilization are not well understo...

The so-called tube-gel method is a sample preparation protocol allowing for management of SDS for protein solubilization through in-gel protein trapping. Because of its simplicity, we assumed that once miniaturized, this method could become a standard for large scale experiments. We evaluated the performances of two variants of the miniaturized version of the tube-gel method based on different ...

membrane assisted liquid extraction(male) technologies are gaining an important role as an extraction /separation technique for actinides and are being deployed as a  promising tool for remediation of nuclear waste generated in the reprocessing plant and other radioactive wastes containing a trace level of radionuclides. the present contribution outlines the classification of membrane assisted ...

in this research, process asymmetric polyetherimide hollow fiber membranes using ethanol (0, 2 and 4 wt%) as non-solvent additive in the polymer dope via phase inversion method were fabricated. aqueous solution of 1-methyl-2-pyrrolidine (nmp) (90%) was applied as a bore fluid to avoid inner skin layer formation and water was used as the external coagulant. the morphology of fabricated membranes...

Solubilization of integral membrane proteins is a process in which the proteins and lipids that are held together in native membranes are suitably dissociated in a buffered detergent solution. The controlled dissociation of the membrane results in formation of small protein and lipid clusters that remain dissolved in the aqueous solution. Effective solubilization and purification of membrane pr...

Solubilization of membrane proteins requires surfactants, whose structural properties play a crucial role in determining the protein phase behavior. We show that ionization of a pH-sensitive surfactant, lauryldymethylamino-N-oxide, bound to the bacterial photosynthetic Reaction Center, induces protein phase segregation in micrometric "droplets." Liquid-liquid phase separation takes place in a n...

A rapid and simple purification of milligram amounts of 2,3-oxidosqualene cyclase, an integral membrane enzyme that catalyzes the cyclization of squalene epoxide to lanosterol, is reported. Several nonionic detergents (Triton X-100, Tween 80, Emulphogene, and lauryl maltoside) were evaluated for solubilization of oxidosqualene cyclase from rat liver microsomes. At a detergent concentration of 5...