the aim of this study was to investigate the effect of the addition of commercial lipase in various forms on the acceleration of the ripening of uf-feta cheese. commercial lipase from aspergillus niger was encapsulated by using silica composition based on sol-gel method. lipase was then added to cheeses in three forms, namely, free lipase, encapsulated lipase, and encapsulated lipase with arabi...

the aim of this study was to investigate the effect of the addition of commercial lipase in various forms on the acceleration of the ripening of uf-feta cheese. commercial lipase from aspergillus niger was encapsulated by using silica composition based on sol-gel method. lipase was then added to cheeses in three forms, namely, free lipase, encapsulated lipase, and encapsulated lipase with arabi...

lipases are versatile biocatalysts with a wide range of application in food, dairy, leather, paper, pharmaceutical and detergent industries.  in this study, mesophilic lipase gene from an indigenous bacillus pumilus f3 that already its gene had been sequenced and  identified, was cloned and expressed in methylotrophic yeast pichia pastoris and codon translation of lipase gene was evaluated in p...

background and objectives: lipases are particularly important due to the fact that they specifically hydrolyze acyl glycerol, oils and greases, which is of great interest for different industrial applications. materialst and methods : in this study, several lipase-producing bacteria were isolated from wastewater of an oil processing plant. the strain possessing the highest lipase activity was i...

Lipases have many applications in biotechnology, thanks to their ability of acylglycerides hydrolysis. They alsp possess the unique feature of acting at the lipid-water interface, which distinguishes them from esterases. Commercially useful lipases are produced by microorganisms with the extracellular lipase being produced by many bacteria including Pseudomonas. The greatest production of lipas...

Background: Lipase enzymes are of great importance in various industries. Currently, extensive efforts have been focused on exploring new lipase producer microorganism as well as genetic and protein engineering of available lipases to improve their functional features. Methods: For screening lipase-producing lactobacilli, isolated strains were inoculated onto tributyrin agar plates. Molecular ...

Cloning and sequencing of a lipase gene from an indigenous Bacillus pumilus, strain F3, revealed an open-reading frame of 648 nucleotides predicted to encode a protein of 215 residues. Sequence analysis showed that F3 lipase contained a signal peptide composed of 34 amino acids with an H domain of 18 residues. A tat-like motif was found in the signal peptide similar to some other Bacillus pumil...

In this study, solid-state fermentation of two types of agricultural residues/products for lipase production in a tray-bioreactor was investigated. Rhizopus oryzae was used as a potential fungus strain and two types of agricultural residues including soybean meal and sugarcane bagasse were utilized as substrate. Fermentation was carried out in two different operational conditions: one with cont...

background: lipase is an enzyme with immense application potential. ester synthesis by lipase catalysis in organic media is an area of key industrial relevance. enzymatic preparations with traits that cater to the needs of this function are hence being intensely researched. objective: the objectives of the study were to immobilize the lipase from bacillus sp. ps35 by cross-linking and adsorptio...

in this study, the effect of addition of three different levels of microbial lipase (0, 4, and 8.0 g lipase in 100 kg milk) was investigated on the physiochemical properties, free fatty acids, textural properties, and microstructure of lighvan cheese during ripening. the addition of lipase did not significantly affect ph, acidity, moisture, and salt content of lighvan cheese, but increased its ...