A new method is described for vital staining of nervous tissue with methylene blue; it involves impregnation of whole embryos or tissue-fragments with the rongalitreduced dye under anaerobic conditions at pH 5, followed by development of the stain in an oxygenated neutral saline solution. Determinations of the solubility of leucomethylene blue and experiments on vital staining with it and with ...

The recently reported vagal nerve stain leucomethylene blue was assessed in 20 patients as an intraoperative aid to attaining complete vagotomy. Of 101 stained specimens removed at surgery, only 30 (30%) contained nerve tissue. Four of 17 postoperative insulin tests were positive by Hollander's criteria. The dye technique was found to be an unreliable method of ensuring complete vagotomy at the...

The severe acute respiratory syndrome caused by COVID-19 is now a global catastrophic event. Currently thereis no approved drug or vaccine for the disease. Methylene blue (MB, oxidized form, blue color) has been used inmany different areas of clinical medicine, ranging from malaria to orthopedics. Leucomethylene Blue (reduced formof MB, colorless) may be applied for the treatm...

Polarization modulation-Fourier transform infrared (PM-FTir) spectra were. obtained in situ for polymer films of neutral red and thionine, and for a lilm of leucomethylene blue generated on a gold electrode as a function of potential. The spectra of the polymer films revealed that the aromatic rings lie mostly parallel to the electrode surface in both the reduced and oxidized forms. In contrast...

Correspondence BJOUr that "after many attempts to find any small intact nerves, the search was abandoned." I am sure that in a case of this nature the dye would have enabled him to find most of these small intact nerves. In a personal communication to me on the use of leucomethylene blue to detect the vagal fibres, A. A. Gunn stated that in 65 truncal vagotomies done in 1970, in 54 of these ope...

Two different methods for the extraction and assay of plastoquinones A, B, C and D from chloroplasts of green plants have been described. The long procedure involves separation of aqueous and lipid phases of extract in a separatory funnel, column chromatography, purification on thin-layer plates, and spectrophotometric assays for quantitative determination of the various plastoquinones. The sho...

To cite: Hadi HIA, Williamson JS, Bhowmick AK. BMJ Case Rep Published online: [please include Day Month Year] doi:10.1136/bcr-2014204986 DESCRIPTION An 85year-old man underwent reversal of loop ileostomy. The integrity of the anastomosis was tested by an injection of methylene blue into the bowel lumen. Postoperatively the patient voided ‘green urine’ and alarmed the nursing staff and junior du...

Methylene blue (MB) was used as a model molecule to characterize the aqueous reactivity of metallic iron in Fe(0)/H(2)O systems. Likely discoloration mechanisms under used experimental conditions are: (i) adsorption onto Fe(0) and Fe(0) corrosion products (CP), (ii) co-precipitation with in situ generated iron CP, (iii) reduction to colorless leukomethylene blue (LMB). MB mineralization (oxidat...