objective: the aim of the present study was the production of recombinant lentviruses that express mir-16. after transduction, altered expression levels of mirna and its target protein were analyzed. methods: a dna fragment that contained the mir-16 precursor was cloned in a lentiviral plasmid. lentiviral vector particles were produced by transient calcium phosphate co-transfection of 293t cell...

Beta catenin gene can inhibit the chondrogenic differentiation of human adipose tissue derived stem cells, while sex determining region Y-box9 promote cells. However, it is still necessary to study role combination two genes in early The purpose this was investigate whether knockdown and overexpression combined with modification cells could Therefore, divided into four groups or lentivirus vect...

The low levels of transduction of human hematopoietic stem cells (HSCs) with Moloney murine leukemia virus (MLV) vectors have been an obstacle to gene therapy for hematopoietic diseases. It has been demonstrated that lentivirus vectors are more efficient than MLV vectors at transducing nondividing cell lines as well as human CD34(+) cells and severe combined immunodeficiency disease repopulatin...

Ruminant lentiviruses share genomic sequences and biologic properties with human immunodeficiency viruses. Four ovine lentivirus strains were assessed for cytopathic effects and virus replication. Lentivirus isolate H/24 produced high virus titers and lysis of synovial cells but replicated slowly and caused no fusion of alveolar macrophages. Lentivirus isolates 84/28 and 85/14 produced low viru...

Feline immunodeficiency virus is a lentivirus of domestic cats that causes significant lifelong infection. Infection with this or similar lentiviruses has been detected in several nondomestic feline species, including African lions (Panthera leo). Although lion lentivirus (FIVple) infection is endemic in certain lion populations in eastern and southern Africa, little is known about its pathogen...

To explore the possible mechanism of KiSS1 in control GnRH secretion participate in sexual development onset and normal reproduction regulation by investigate the changes of expression of KiSS1, GnRH in hypothalamus and LH, FSH, E2 in serum,by using RNA interference Mediated with Lentivirus-based Vectors, after Interfering expression of KiSS1. Constructed the eukaryotic expression plasmid of ra...

BACKGROUND Aseptic loosening is a significant impediment to joint implant longevity. Prosthetic wear particles are postulated to play a central role in the onset and progression of periprosthetic osteolysis, leading to aseptic loosening of the prosthesis. METHODS We investigated the inhibitory effects of a lentivirus-mediated short hairpin RNA that targets the TNF-alpha gene on the particle-i...

Small ruminant lentivirus (SRLV) is a group of viruses the Retroviridae family, shared between caprine, ovine and wild ruminants. It responsible for systemic infection that can affect lungs, central nervous system, mammary gland joints, causing chronic, insidious, progressive diseases, seriously affecting animal health. Concurrently, it associated with decrease in milk production, leading to ma...