Background: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. Aims: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G;) stain for avian blood smears and to compare its efficacy with conventional staining methods. Methods:<...

In recent years it has become increasingly difficult to purchase ingredients for the preparation of various modifications of Romanowsky stains, such as Giemsa, Leishman and Wright's, at a reasonable cost; if indeed they can be obtained at all. During the past year, work has been in progress in the laboratories of the Malaria Institute of India with the object of producing a stain in which readi...

Rapid diagnosis is a prerequisite for institution of effective treatment and reducing the mortality and morbidity of falciparum malaria. This study was taken up to compare the efficacy of various rapid methods viz, acridine orange, Plasmodium falciparum histidine rich protein II antigen detection and Field's stain with traditional microscopy i.e, Leishman stain for diagnosing falciparum malaria...

LD bodies are identified by light microscopy of the specimen obtained after staining with Giemsa or Leishman stain. The sensitivity of splenic aspirate smear is more than 95% and is regarded as the gold standard for the diagnosis of Kala-azar. But the sensitivity of bone marrow smear is only about 60-85% and the procedure is painful whereas splenic aspiration carries the risk of severe hemorrha...

BACKGROUND This study was conducted to explain the trend of Cutaneous Leishmaniasis (CL) in Kermanshah Province, western Iran from 1991-2012. METHODS In this analytical-descriptive study, all of the patients suspected to CL in Kermanshah Province were studied. Smears were prepared from most of them, stained with Giemsa, and examined microscopically for Leishman bodies. A few of the patients w...

The stains commonly used for staining blood films (Giemsa's, Wright's, Leishman's, etc.) have become expensive, and in many cases difficult to obtain. In order to get the best results with these stains, acetone-free alcohol and distilled water of a specified pH are necessary. This again increases costs and complicates technique. Furthermore, films, and particularly thick-drop slides, are usuall...

Introduction:Fine Needle Aspiration Cytology (FNAC) is used widely for the preliminary pre-operative diagnosis of all superficial palpable lesions and for deep seated lesions under ultrasound guidance. Though Pap stain, May GrunwaldGiemsa (MGG) and Haematoxylin and Eosin (H & E) stains are used commonly, Leishman stain provides good cytoplasmic and background details. Hence, it can beused in ad...