the aim of the clinical laboratory in endocrine disease diagnosis and management is detecting either the hormones or their downstream reaction metabolites or some other related substances. in the case of hormone measurement almost all the routine methods are based on immunoassay with different labels (radioimmunoassay, enzyme linked immunosorbant assay, chemiluminescence assays …) and different...

direct agglutination test (dat) is a sensitive and simple method, which is applicable in field and unequipped laboratories. in order to evaluate dat in the diagnosis of toxoplasma infections, the antigen was prepared in the protozoology unit, school of public health and it was used in the following studies. a- 251 serum samples collected from suspected toxoplasmosis out-patients were examined f...

currently, different highly sensitive nucleic acid detection-based protocols used in medical diagnostic laboratories need specific working conditions, and if no optimization is performed, misleading results can be obtained. limitations of each protocol is different in obtaining an appropriate result. awareness of these limitations plays an important role in providing a reliable result for physi...

Methylmalonic acidemia (MMA) is usually caused by a deficiency of the enzyme methylmalonyl-CoA mutase (MCM), a defect in the transport or synthesis of its cofactor, adenosyl-cobalamin (cblA, cblB, cblC, cblF, cblD, and cblX), or deficiency of the enzyme methylmalonyl-CoA epimerase. A comprehensive diagnostic approach involves investigations of metabolites with tandem mass spectrometry, organic ...

methods genomic dna of cultured s. agalactiae was prepared and loop-mediated isothermal amplification (lamp) primers were designed based on the camp gene in bacteria. the optimum primer set was selected based on the reaction speed and specificity. the reaction result was monitored visually. the sensitivity and specificity of the lamp method were evaluated and compared with polymerase chain reac...