OBJECTIVES The lactulose:mannitol (L:M) diagnostic test is frequently used in field studies of environmental enteropathy (EE); however, heterogeneity in test administration and disaccharide measurement has limited the comparison of results between studies and populations. We aim to assess the agreement between L:M measurement between high-performance liquid chromatography with pulsed amperometr...

BACKGROUND Plasma Citrulline concentration has been correlated to functional enterocyte mass. Dried blood spot (DBS) analysis using LC-MSMS reduces sample amount needed. We optimized DBS elution to increase precision and accuracy of DBS LC-MSMS analysis. METHOD DBS control samples were eluted in varying pH (2.2-7.0) and for varying times (15-75 min) and Cit, Arg and Orn were analyzed using LC...

Efficient strategies are required to implement comprehensive suspect screening methods using high-resolution mass spectrometry within environmental monitoring campaigns. In this study, both liquid and gas chromatography time-of-flight mass spectrometry (LC-QTOF-MS and GC-QTOF-MS) were used to screen for >5000 target and suspect compounds in the Sacramento-San Joaquin River Delta in Northern Cal...

Lysine-specific chemical crosslinking in combination with mass spectrometry is emerging as a tool for the structural characterization of protein complexes and protein-protein interactions. After tryptic digestion of crosslinked proteins there are thousands of peptides amenable to MSMS, of which only very few are crosslinked peptides of interest. Here we describe how the advantage offered by off...

BACKGROUND Although vitamin D insufficiency is prevalent in the community, only a few population-based studies have measured serum 25-hydroxy-vitamin D (25OHD) levels during pregnancy and in newborns. Maternal vitamin D deficiency has been linked to pregnancy complications, as well as hypocalcaemia and rickets in the newborn. Here, the authors report third-trimester maternal and newborn-serum 2...

The use of ion mobility separations (IMSs) in metabolomics approaches has started to be deeply explored the last years. In this work, liquid chromatography (LC) coupled IMS-quadrupole time-of-flight mass spectrometry (QTOF MS) been evaluated a experiments using single injection samples. IMS allowed obtaining cleaner fragmentation spectra, nearly tandem MS quality, data-independent acquisition m...

An improved online two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF MS) system was developed for the lipid profiling of human plasma, in which different lipid classes were separated by the first dimensional normal-phase (NP) LC and different lipid molecular species were separated by the second dimensional reversed-phase (RP) LC. This 2D LC-QToF MS sy...

background: this study aimed to survey a total five common human and veterinary antibiotics, including ampicillin, oxytetracycline, enrofloxacin, tylosin, and ciprofloxacin based on spe-lc-ms-ms technology in a wastewater treatment plant at central plateau of iran. also two sampling techniques, passive and grab samplings were compared in the detection of selected antibiotics. methods: in februa...

Guggulu (Commiphora wightii oleo-gum resin) is exported in more than 42 countries including developed like the United Kingdom and States of America. The demand for guggulu its production. Various purification processes have been mentioned Ayurvedic classical literature which are not explored well yet, so it impossible to discriminate between purified raw guggulu. In present study, an effort was...

The purpose of this study was to identify porcine-specific peptide markers from thermally processed meat that could differentiate pork from beef, chevon and chicken meat. In the initial stage, markers from tryptic digested protein of chilled, boiled and autoclaved pork were identified using LC-QTOF-MS. An MRM method was then established for verification. A thorough investigation of LC-QTOF-MS d...