The importance of coordinating cell growth with proliferation has been recognized for a long time. The molecular basis of this relationship, however, is poorly understood. Here we show that the ribosomal protein L23 interacts with HDM2. The interaction involves the central acidic domain of HDM2 and an N-terminal domain of L23. L23 and L11, another HDM2-interacting ribosomal protein, can simulta...

Escherichia coli ribosomal protein L23 was derivatized with [3H]2, 4-dinitrofluorobenzene both at the N terminus and at internal lysines. Dinitrophenyl-L23 (DNP-L23) was taken up into 50 S subunits from a reconstitution mixture containing rRNA and total 50 S protein depleted in L23. Unmodified L23 competed with DNP-L23 for uptake, indicating that each protein form bound in an identical or simil...

Benjakul have also been used for cancer therapy by folk medicine. The ethanolic extracts, five fractions and three pure compounds from Benjakul were investigated for cytotoxic activities against two types of human lung cancer cell lines (COR-L23 and A549) and one normal human lung myofibroblasts (MRC-5). The cytotoxic activities of the BEN-3 fraction which fractionated with vacuum liquid chroma...

NK cell function in the rat is only defined in a rudimentary way due to missing tools for clear NK cell identification. The present study introduces the congenic LEW.BH-NKC rat strain which allows distinct detection of rat NK cells using commercial antibodies. LEW.BH-NKC rats were exposed in vivo to the porcine B cell line L23 by subcutaneous transfer of L23 cell suspension. We used Luciferase ...

1 2 3 4 5 6 7 8 1 e0 e9 e11 e12 e10 e7 e15 2 e4 e2 e5 e6 L L L e13 3 e1 e3 e8 L L L L e16 4 L L L L L L e17 5 e0 e11 e12 e10 e9 L e15 6 e4 e2 e6 L19 e7 e5 L20 e13 7 e1 e3 L24 e8 L23 L22 e16 8 L25 L26 L30 L27 L28 L29 e17 9 e0 e12 e10 L31 e11 e9 e15 10 e4 e2 L19 L20 e6 e7 e13 11 e1 e3 L22 L23 L24 e8 e16 12 L L25 L26 L29 L30 L27 L28 e17 13 e0 e10 L21 e12 e9 e11 e15 14 e4 e2 e7 e6 L20 L19 e13 15 e1...

The region of the large-subunit rRNA encompassing the D7 divergent domain is organized within eukaryotes in a patchwork of short conservative secondary-structure features interspersed with more rapidly evolving sequences. It contains the attachment site of protein L25 (E. coli L23), which binds rRNA in the first stages of ribosome assembly, suggesting a crucial importance of this region in ribo...

It has been demonstrated that embryonic chicken gizzard smooth muscle contains a unique embryonic myosin light chain of 23,000 mol wt, called L23 (Katoh, N., and S. Kubo, 1978, Biochem. Biophys. Acta, 535:401-411; Takano-Ohmuro, H., T. Obinata, T. Mikawa, and T. Masaki, 1983, J. Biochem. (Tokyo), 93:903-908). When we examined myosins in developing chicken ventricular and pectoralis muscles by t...

As newly synthesized polypeptides emerge from the ribosome, they interact with chaperones and targeting factors that assist in folding and targeting to the proper location in the cell. In Escherichia coli, the chaperone trigger factor (TF) binds to nascent polypeptides early in biosynthesis facilitated by its affinity for the ribosomal proteins L23 and L29 that are situated around the nascent c...

In eukaryotes, newly synthesized proteins interact co-translationally with a multitude of different ribosome-bound factors and chaperones including the conserved heterodimeric nascent polypeptide-associated complex (NAC) and a Hsp40/70-based chaperone system. These factors are thought to play an important role in protein folding and targeting, yet their specific ribosomal localizations, which a...