Several proline derivatives such as L-azetidine-2-carboxylic acid, cis-4-hydroxy-L-proline, and 3,4-dehydro-DL-proline prevent procollagen from folding into a stable triple-helical conformation, thereby reducing excessive deposition of collagen in fibrotic processes and the growth of tumors. This study was performed to investigate whether the recently discovered human proton-coupled amino acid ...

L-Proline can be used by Bacillus subtilis as a sole source of carbon or nitrogen. We traced L-proline utilization genetically to the putBCP (ycgMNO) locus. The putBCP gene cluster encodes a high-affinity proline transporter (PutP) and two enzymes, the proline dehydrogenase PutB and the Δ(1)-pyrroline-5-carboxylate dehydrogenase PutC, which jointly catabolize L-proline to L-glutamate. Northern ...

Uptake of L-proline, hydroxy-L-proline and 5-oxo-L-proline by luminal-membrane vesicles isolated either from whole cortex or from pars convoluta or pars recta of proximal tubules was studied by a spectrophotometric method. Uptake of L-proline and hydroxy-L-proline by vesicles from whole cortex was mediated by both Na+dependent and Na+-independent, but electrogenic, processes, whereas transport ...

The mechanism by which L-proline triggers germination in Bacillus megaterium QM B1551 spores was investigated. First, brief exposure of spores to L-proline, followed by dilution, was sufficient to trigger germination. Once germination was triggered, the spores continued initiation of germination and did not require high concentrations of L-proline. Triggering of germination was pH and temperatu...

Hydroxy-L-proline was first isolated by Fischer in 1902 from a hydrolysate of gelatin (l).l The synthesis and determination of the structure were reported by Leuchs and coworkers (5--g), and, in 1919, the separation of the two racemic stereoisomers and the resolution of each racemate through mixtures of quinine with the corresponding N-phenylisocyanate derivatives were accomplished (5). In the ...

During the fermentation of sake, cells of Saccharomyces cerevisiae are exposed to high concentrations of ethanol, thereby damaging the cell membrane and functional proteins. L-proline protects yeast cells from damage caused by freezing or oxidative stress. In this study, we evaluated the role of intracellular L-proline in cells of S. cerevisiae grown under ethanol stress. An L-proline-accumulat...

The findings (a) stereospecific counter transport; (b) equilibrium uptake of L-proline decreased with increasing medium osmolarity; and (c) L-proline and Na+ were taken up into identical intravesicular spaces, indicate that uptake of L-proline by rabbit renal brush border membranes represents transport into membrane vesicles, not surface binding to the membrane. An Na+ gradient between extraves...