background:   h2-eb1 molecule which is the homolog of human hla-drb1 is proposed to be associated with allergic rhinitis (ar). construction of   h2-eb1 knockout animal models provides a tool to elucidate the role of h2-eb1 and ar pathogenesis. objective:   to establish the h2-eb1 knockout model and investigate the h2-eb1 functions in   h2-eb1 knockout mice as a model of ar. methods: the cre/lox...

Background:   H2-EB1 molecule which is the homolog of Human HLA-DRB1 is proposed to be associated with allergic rhinitis (AR). Construction of   H2-Eb1 knockout animal models provides a tool to elucidate the role of H2-EB1 and AR pathogenesis. Objective:   To establish the H2-Eb1 knockout model and investigate the H2-EB1 functions in   H2-Eb1 knockout mice as a model of AR. Methods: The Cre/Lox...

It has long been established that histamine plays a role as a mediator of inflammation. From numerous studies, it has been well known that the amine has many pharmacological actions on a variety of organs. To evaluate the role of histamine in pain perception, we generated HDC knockout mice using a gene targeting method. Histamine is a hydrophilic autacoid, and in most tissues it is stored and s...

We consider the problem of using simulation to efficiently estimate the win probabilities for participants in a general random knockout tournament. Both of our proposed estimators, one based on the notion of “observed survivals” and the other based on conditional expectation and post-stratification, are highly effective in terms of variance reduction when compared to the raw simulation estimato...

It has long been established that histamine plays a role as a mediator of inflammation. From numerous studies, it has been well known that the amine has many pharmacological actions on a variety of organs. To evaluate the role of histamine in pain perception, we generated HDC knockout mice using a gene targeting method. Histamine is a hydrophilic autacoid, and in most tissues it is stored and s...

embryonic stem cells (escs) are derived from the inner cell mass (icm) of blastocyst and differentiate into all three embryonic germ layers: ectoderm, endoderm, and mesoderm. in this study, escs are derived from hand made cloning (hmg) blastocysts and their efficiencies compared to escs derived from in vitro fertilization (ivf) embryos. feeder layer was used for escs culture, and culture medium...

The deeper we look at human genomes, the more unexpected things we find. And increasingly we are surprised by what each of us lacks. In particular, in recent years we have learned that naturally occurring human gene knockouts are prevalent. The results of the Exome Aggregation Consortium (ExAC) now paint a rich and detailed picture of gene redundancy and essentiality in humans (Lek et al., 2016...