Root influx parameters (Vmax and Km) of wheat (Triticum aestivum var. HD-2285) were determined at different stages of crop growth, viz. CRIS-Crown Root Initiation Stage, MTS-Maximum Tillering Stage, FLS-Flag Leaf Stage and DFS-Dough Formation Stage. Wheat was grown in sand medium with Hoagland nutrient solution (all nutrients except potassium). Plants were taken out from sand culture at 22, 41,...

abstract        background and objective: peroxidases catalyze protein oxidation and lipid peroxidation. the activity of these enzymes in nerve cells is involved in causing disorders such as alzheimer's and parkinson's disease. this study investigated the effect of citrus aurantium, foeniculum vulgare and rosmarinus officinalis essential oils on activity of peroxidase enzyme.       me...

in present study, the kinetic parameters of purified trypsin from pyloric caeca of common kilka (c.cultriventris caspia) were measured using the amidolytic (bapna) and esterolytic (tame) substrates. the results of the kinetic comparison between bapna and tame substrates showed that kinetic parameters including maximum velocity (vmax), catalytic constant (kcat) and catalytic efficiency (kcat/km)...

Organophosphorus pesticides (OPs) are a public health concern due to their worldwide use and documented human exposures. Phosphorothioate OPs are metabolized by cytochrome P450s (P450s) through either a dearylation reaction to form an inactive metabolite, or through a desulfuration reaction to form an active oxon metabolite, which is a potent cholinesterase inhibitor. This study investigated th...

In order to distinguish between the Ping-Pong and sequential mechanisms of cation transport in the cardiac Na(+)-Ca2+ exchange system, the initial rates of the Nai-dependent 45Ca uptake (t = 1 s) were measured in reconstituted proteoliposomes, loaded with a Ca chelator. Under "zero-trans" conditions ([Na]o = [Ca]i = 0) at a fixed [Na]i = 10-160 mM with varying [45Ca]o = 2.5-122 microM for each ...

The substrate recognition determinants of Ca2+/calmodulin-dependent protein kinase Ia were investigated by using peptide analogues based on the amino acid sequence around Ser-9 of synapsin I. The Km and Vmax for the synthetic peptide Leu-Arg-Arg-Arg-Leu-Ser-Asp-Ala-Asn-Phe are 3.9 microM and 18.5 mumol/(min.mg), respectively. Deletion of Leu at the -5 position lowers the Vmax/Km by 470-fold. Th...