kinetic studies were made of the reactions between triphenylphosphine 1, dialkyl acetylenedicarboxylates 2 in the presence of nh-acid, such as maleimid (as a protic/nucleophilic reagent) 3. to determine the kinetic parameters of the reactions, they were monitored by uv spectrophotometery. the second order fits were automatically drawn and the values of the second order rate constant (k2) were a...

1. Kinetic measurements of the forward reaction catalysed by ATP-galactose phosphotransferase were carried out with a purified preparation from pig liver. 2. The rate of reaction at pH7.8 is dependent on the concentration of MgATP(2-) rather than total ATP or magnesium chloride concentrations. 3. The effect of changes in pH on K(m) (galactose), K(m) (MgATP(2-)) and V(max.) was studied. 4. Of se...

The mechanism of calf brain hexokinase has recently been studied in our laboratory (1). It was concluded from kinetic experiments that adenosine diphosphate (ADP) dissociates from the enzyme before addition of substrate glucose. This hypothesis was supported by kinetic studies of n-mannose, ADP, and glucose g-phosphate inhibition. The experiments appear to lend credence to theories in which an ...