in this study, immunohistochemistry was used to clarify the early stages of viral kinetics and cycliccourse of ibdv, ir499, which has been described earlier as a very virulent strain (vvibdv). fifteen, 4-week-old spf chickens were inoculated with 10³ eid50 of vvibdv, ir499, via oculo/nasal route. five birdsserved as controls, and inoculated with phosphate buffered saline (pbs). the birds were t...

infectious bursal disease virus (ibdv) infects young chickens and causes serious lose to the poultry industry, worldwide. previous attempts using purified bacterially expressed ibdv vp2 failed to elicit a protective immune response to the virus. this study was designed to investigate if the initial expressed protein contained neutralizing epitopes but became nonfunctional during purification st...

increased susceptibility of birds to avian pathogens in intensive husbandry system has emphasized on necessity of improvement of innate and specific immune responses of birds by the fast establishment of a beneficial microflora and immune stimulator factors to guarantee healthy and low-price products. during this study, 192 one-day-old broiler chicks (ross-380) in four groups with three replica...

various commercial vaccines for immunization of broiler chickens against infectious bursal disease (ibd) are available, so it would be appropriate to compare the pathogenicity and immune response of chickens to these vaccines. in this study the pathogenicity and serologic response of four ibd vaccines, cloned d78®, bursine-2®, bursimune® and cevac gambo-l®, were evaluated in specific pathogen f...

the pathogenicity of a recent isolate of infectious bursal disease virus (ibdv), ir499, isolated from a nonvaccinated flock with 17.5 % mortality rate in susceptible spf chickens, chickens embryos and broilers was discussed. the molecular characterization of the virus based on the restriction fragment length polymorphism (rflp) pattern was also investigated. the mortality rates were 85% and 22%...

infectious bursal disease (ibd) is a highly contagious disease of chickens caused by the infectious bursal disease virus (ibdv). this study was conducted to characterize three ibdv strains from iran. a reverse transcriptase-polymerase chain reaction (rt-pcr) procedure was used to amplify a 715-bp fragment of the vp1 gene from ibdv strains. amplified vp1 fragments of the three iranian ibdv strai...

background and aims: infectious bursal disease (ibd) is an acute contagious viral disease of birds worldwide. the causative virus induces a persistent immune suppression following destroy b lymphocytes precursors in bursal lymphoid follicles. vaccination is the main strategy for prevention of the disease in commercial poultry industry. materials and methods: to produce a live vaccine against th...

infectious bursal disease (ibd), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (ibdv). to improve the immunogenicity of recombinant ibdv subunitvaccine, an attempt was made to find a new way to prepare ibd vaccine containing glycosylated mvp2antigen. firstly, ibdv mvp2 gene (with a nucleic acid sequence encoding b cell epitope of ibdv(...

Infectious Bursal Disease is a severe viral disease of chicken responsible for serious economic losses to poultry farmers. The causative agent, Infectious Bursal Disease virus, is inhibited by nitric oxide. Root extract of the Indian ginseng, Withania somnifera, inhibits Infectious Bursal Disease virus in vitro. Also, Withania somnifera root extract is known to induce nitric oxide production in...