indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of h. contortus. plate elisa was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. indirect plate elisa was able to demonstrate...

Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...

The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR ...

this paper presents the bending analysis of annular plates by the indirect trefftz boundary approach. the formulation for thin and thick plates is based on the kirchhoff plate theory and the reissner plate theory. the governing equations are therefore a fourth-order boundary value problem and a sixth-order boundary value problem, respectively. the trefftz method employs the complete set of solu...

background: considering that elisa method presently is the test of choice for diagnosis of fasciolo­sis, the present study was undertaken to evaluate the maximum validity of coated plates at dif­ferent temperatures and different times during one year of evaluation. methods: serum samples of patients infected with fasciolosis (n=10), hydatidosis (n=5), toxocaria­sis (n=5), and negative control s...

In previous studies, polyclonal antibodies against the organophosphorus insecticide fenthion were obtained and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for this pesticide. In this study, using these antibodies and an enzyme tracer, direct competitive ELISAs for fenthion in microtiter plate and dipstick formats were developed. The microtiter plate ELISA sho...

PURPOSE The purpose of this study was to investigate the seroprevalence of brucellosis among high-risk group individuals, consisting of veterinarians and para-veterinarians, shepherds, butchers and animal owners. METHODS The present work was carried out at Project Directorate on Animal Disease Monitoring and Surveillance, Bangalore, by using the recently developed indirect enzyme-linked immun...