background: the refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. objectives: the purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produc...

background: the expression of mouse tumor necrosis factor alpha (tnf-α) in escherichia coli is a favorable way to get high yield of protein; however, the formation of cytoplasmic inclusion bodies, which is the consequence of insoluble accumulated proteins, is a major obstacle in this system. to overcome this obstacle, we used a pulsed dilution method to convert the product to its native conform...

THE epithelial-cell inclusion, which is characteristically found in the early stages of trachoma was first described by Halberstaedter and Prowazek (1907). Since that time, a considerable literature concerning the nature of these bodies has accumulated. It is generally conceded that they consist of virus particles together with material elaborated by the virus and/or produced by the parasitized...

background: production of tissue plasminogen activator protein (t-pa) in prokaryotes systems has many problems such as the lack of active protein production, multiple purification steps, and renaturation process which has been shown to be costly and time-consuming. methods: in this study, reteplase which is the nonglycosylated active domain of t-pa was used to transform top10 escherichia coli (...

The formation of inclusion bodies (IBs) constitute a frequent event during the production of heterologous proteins in bacterial hosts. Although the mechanisms leading to their formation are not completely understood, empirical data have been exploited trying to predict the aggregation propensity of specific proteins while a great number of strategies have been developed to avoid the generation ...

For an arbitrary second-order stable matrix A, we calculate the maximum positive value R for which the differential inclusion ẋ ∈ FR(x) := {(A +∆)x,∆ ∈ R2×2, ‖∆‖ ≤ R} is asymptotically stable.