results in this study, seven sirna molecules were rationally designed against the nucleoprotein gene and validated using various computational methods for silencing different strains of rsv. additionally, three effective sirna molecules targeting the overlapping region of m2/l mrna were designed. conclusions this approach provides insight and a validated strategy for chemical synthesis of an an...

in this work, we reported the synthesis and evaluation of antimycobacterial and antifungalactivity of a series of thiocarbohydrazone derivatives which are thiacetazone congeners. thetarget compounds were synthesized in superior yields by reacting thiocarbohydrazide withdifferent aromatic aldehydes and methyl ketones. compounds 8, 19 and 25 were found to bethe most potent derivatives, exhibiting...

hirudin is an anticoagulant agent of the salivary glands of the medicinal leech. recombinant hirudin (r-hir) displays certain drawbacks including bleeding and immunogenicity. to solve these problems, cysteine-specific pegylation has been proposed as a successful technique. however, proper selection of the appropriate cysteine residue for substitution is a critical step. this study has, for the ...

background: sigma factors are proteins that regulate transcription in bacteria. sigma factors can be activated in response to different environmental conditions. the rpos (rna polymerase, sigma s) gene encodes sigma-38 (σ38, or rpos), a 37.8 kda protein in pseudomonas aeruginosa (p. aeruginosa) strains. rpos is a central regulator of the general stress response and operates in both retroactive ...

objective(s): tyrosinase is a key enzyme in pigment synthesis. overproduction of melanin in parts of the skin results in hyperpigmentation diseases. this enzyme is also responsible for the enzymatic browning in fruits and vegetables. thus, its inhibitors are of great importance in the medical, cosmetic and agricultural fields. materials and methods: a series of twelve kojic acid derivatives wer...

introduction: omp31 is animmunodominant and protective antigen conserved in brucella species and a good candidate for vaccine design. material & methods: the present study aimed at in silico design of the truncated omp31 (tomp31) using bioinformatic tools and to express the selected form in escherichia coli (e. coli) results and conclusion: various bioinformatically calculated scores for the mo...

objective(s): study of non-coding rnas is considerable to elucidate principal biological questions or design new therapeutic strategies. mirnas are a group of non-coding rnas that their functions in pi3k/akt signaling and apoptosis pathways after t cell activation is not entirely clear. herein, mirnas expression and their putative targets in the mentioned pathways were studied in the activated ...

background: neisseria meningitidis, a life-threatening human pathogen with the potential to cause large epidemics, can be isolated from the nasopharynx of 5–15% of adults. the aim of the current study was to evaluate biophysical and biochemical properties and immunological aspects of chimeric acyl-carrier protein-macrophage infectivity potentiator protein-type iv pilus biogenesis protein antige...

materials and methods in silico analysis of the cadf gene was carried out by several software and available online tools. a duplex pcr optimized with specific primers was used for detection and differentiation of both species. to evaluate specificity and sensitivity of the test, a panel of different campylobacter spp. together with several intestinal bacterial pathogens was tested. the limit of...

MOTIVATION We developed a technique and a tool for degenerate primer design based on multiple local alignments employing the MEME algorithm supported with electronic PCR. The objective is to find adequate primers starting from sequences with poor global similarity. We show an example of its application in our laboratory to find sequences in Brachiaria with similarity to ESTs related to apomixis.