Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasm...

BACKGROUND Colorectal, lung and breast tumors are the most devastating and frequent malignances in clinical oncology. SEREX-analysis of colon cancer leads to identification of more than hundred antigens which are potential tumor markers. With idea that immunoscreening with pool of allogeneic sera is more productive for antigen isolation, SEREX-analysis was applied to four cases of stages II-IV ...

The etiological agent of most cases of post-transfusion hepatitis and a variable proportion of sporadic non-A, non-B hepatitis was discovered in 1989, by recombinant cDNA immunoscreening of serum from a chimpanzee chronicaIIy infected with a contaminated human factor VIII concentrate (Choo et al., 1989). The agent was termed hepatitis C virus (HCV). It is a positive-strand RNA virus distantly r...

Although the cDNA of human decay accelerating factor (DAF) which restricts complement activation on homologous cell membranes was cloned in 1987, all trials to detect the cDNA of mouse DAF by cross-hybridization were unsuccessful. However, by immunoscreening with a rabbit antiserum against purified mouse DAF, we successfully cloned the cDNA. It contains four typical short consensus repeats (SCR...

The identification of human cancer antigens has opened new approaches to the development of cancer vaccines. Characterization of the defined antigens including mRNA expression analysis, protein analysis, antibody response and epitope identification are important to assess the value of those antigens.The aim of the present work is to identify tumor specific or tumor associated antigens by serolo...