background: monoclonal antibodies have been employed extensively for the identification of leishmania species, development of diagnostic tests and in the characterization of defined leishmanial antigens. objectives: identification and characterization of  leishmania spp. directly from cutaneous lesions of infected individuals. methods: an immunoperoxidase test (avidin-biotin technique) using mo...

background: vitiligo is a dermatological disorder of unknown etiology with a common incidence in southern iran. presence of autoantibodies to melanocyte antigens suggested an autoimmune basis of the disease. objective: in this study, the presence of rheumatoid factor (rf) in sera and skin biopsies of vitiligo patients was investigated. methods: the presence of rf in sera of 35 vitiligo and 32 n...

Azure A and methylene blue ("Diff-Quik," DQ) and tissue urease (U) tests are popular methods to diagnose Helicobacter pylori. These tests usually correlate well but sometimes produce discordant results. This study evaluates the DQ and U tests by comparing them with the immunoperoxidase reference method to resolve discordant results. DQ and U tests were performed on gastric biopsies. Results wer...

background: different methods have been used for characterization of leishmania promastigotes. monoclonal antibodies are useful in characterization of   leishmania spp . both amastogotes and promastigotes. objective: comparing the characterization of   leishmania spp. promastigotes with immunoperoxidase test (avidin-biotin) techniques and an indirect immunofluorescent assay (ifa).   methods: ap...

The indirect immunoperoxidase technique with type-specific antisera was compared to kinetic neutralization for the typing of herpes simplex virus strains. There was complete agreement between the results obtained by kinetic neutralization and indirect immunoperoxidase. The indirect immunoperoxidase assay was far simpler to perform and interpret than the kinetic neutralization tests, and it offe...

The indirect immunoperoxidase method was applied to the rapid diagnosis of mucocutaneous herpes simplex virus infection. Specimens were obtained directly from lesions at various stages in their evolution. The immunoperoxidase method identified herpes simplex virus-infected cells in 91% of vesicular lesions which were positive by standard virological culture. At later stages, the number of cases...

Background: Different methods have been used for characterization of Leishmania promastigotes. Monoclonal antibodies are useful in characterization of   Leishmania spp . both amastogotes and promastigotes. Objective: Comparing the characterization of   Leishmania spp. promastigotes with immunoperoxidase test (Avidin-Biotin) techniques and an indirect immunofluorescent assay (IFA).   Methods: Ap...