as igm and iga-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of igm and iga enriched immunoglobulins. fraction iii which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using sephadex deae a-50 and 0.1 m tris-d.35m naci buff...

otitis media with effusion (ome) is very common in pediatric patients. immune reactions in serum and middle ear system play roles in the etiology, pathogenesis and prevention of otitis media. immunologically active antigens interact with immunocompetent cells in the lamina propria of the middle ear to produce a local immune response. in this investigation, 32 sera and 50 middle ear fluid sample...

different classes of autoantibodies against two antigenic forms of modified low density lipoprotein (mod-ldl) were detected in 140 patients by indirect elisa method. investigated autoantibodes included total immunoglobulins (igt). immunoglobulin g (igg), and immunoglobulin m (igm) against oxidized ldl with copper ions (ox-ldl) and modified ldl with malondialdehyde (mal-ldl) (igt-o) igt against ...

Basic research in the chemistry of hexameric resorcin[4]arenes and pyrogallol[4]arenes during the last decade is reviewed. Applications of NMR methods to determine solution structures, host guest properties and exchange dynamics are discussed. The scientific issue is the behavior of molecules in small spaces; the challenge is to translate this information to practical applications in, say, cata...

Chronic lymphocytic leukemia cells with relatively large amounts of mu and kappa immunoglobulin structures on the surface, and apparently very small amounts intracellularly, were subjected to homogenization or washing after freezing and thawing. After a light centrifugation, which sedimented the nuclei and unbroken cells, most of the immunoglobulin structures were found in the supernatant. Ultr...

immunoglobulin class switch recombination deficiencies (ig csr deficiencies) or hyper igm syndromes (higm) are a group of primary immunodeficiency diseases, characterized by defective cd40 signaling of b cells resulting into a csr and a somatic hypermutation. the affected patients are characterized with reduced serum levels of igg and iga, and normal or elevated level of igm, which lead to incr...

Lactate dehydrogenase (LDH) derived from rabbit muscle enhanced IgM production by human-human hybridoma HB4C5 cells 12.4-fold at 320 mug/ml under serum-free conditions. LDHs from pig muscle and pig heart also accelerated IgM production 8.4- and 6.4-fold, respectively. The immunoglobulin production stimulating activity of LDH was not accompanied by activation of cell proliferation. LDH from rabb...

Proteins of purified rubella virus were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and immunoblotted with human sera and immunoglobulin class heavy-chain-specific peroxidase conjugates. The levels of rubella antibodies in these sera were predetermined by the radial hemolysis test, the density gradient centrifugation method for immunogl...

background : cytomegalovirus (cmv) is the most common cause of congenital infection, with increased morbidity and mortality at birth. the risk of intrauterine infection largely depends on the time of maternal infection during pregnancy. currently, no national screening test for cmv infection is available during pregnancy in iran. the aim of this study was to determine the seroprevalence of cmv ...

Immunoglobulin M (IgM) is the primary antibody in fish, which transported from epithelial cells into external secretion system by polymeric immunoglobulin receptor (pIgR). In this study, full length of IgM and pIgR complementary DNA sequences leopard coral grouper (Plectropomus leopardus) was characterized, their expression levels under Vibrio harveyi infection were studied. cDNA sequence analy...