since the incidence of symptomatic congenital cytomegalovirus infection is low (~0.05%) and risk factors are not well defined, it is difficult to develop strategies for prevention. the aim of this study was to recognise the utility of specific immunoglobulin g avidity analysis for distinguishing primary infection from past/recurrent infection. sera from 50 women with cytomegalovirus specific im...

background and aims: toxoplasmosis is a parasitic disease which may cause some laboratory symptoms in infected individuals. one of the main ways of transmition this organism is placenta to fetus pathway. if this transmission occurs in the 3th month of pregnancy, the abortion, central nerve system and ocular disorder will happen. because of this issue, the precise technique for detection of toxo...

laboratory diagnosis of acute measles is usually achieved by serology assays for measle-specific igm antibody. for comparison of measle-specific igm antibody in saliva and serum, 95 paired blood and saliva samples were collected 1-14 days after the onset of rash. the specimens were tested for specific igm antibody by an igm antibody-capture enzyme immunoassay (eia). measles igm antibody was det...

Background: Toxoplasmosis is a zoonotic disease with high seroprevalence worldwide. Several immunological methods have been described for diagnosis of toxoplasmosis. Objective: To determine the parasitemia period in patients infected with toxoplasma using PCR and comparing serological data with molecular results. Methods: 154 serum samples from patients with toxoplasmosis were examined. Presenc...

background: the prevalence of toxoplasma gondii infection in the blood donors has been poorly studied. the aim of this study was to assess the prevalence of acute and chronic toxoplasmosis in blood products. methods: a total of 250 blood products (112 fresh frozen plasma and 138 packed cells) in the blood transfusion institute, shiraz, iran were tested for spe-cific t. gondii antibodies (igg an...

Background Beta-thalassemia patients receive blood products from blood transfusion centers repeatedly. Blood transfusion can transmit Cytomegalovirus (CMV) and Toxoplasma gondii. The aim of this study was serological evaluation of these two infectious agents in thalassemia patients. Materials and Methods In a cross-sectional study, the enzymelinked immunosorbent assay (ELISA) testing was pe...

background: toxoplasmosis is a zoonotic disease with high seroprevalence worldwide. several immunological methods have been described for diagnosis of toxoplasmosis. objective: to determine the parasitemia period in patients infected with toxoplasma using pcr and comparing serological data with molecular results. methods: 154 serum samples from patients with toxoplasmosis were examined. presenc...

We used hemadsorption immunosorbent technique (HIT) to detect mumps immunoglobulin M (IgM) antibody. IgM from human sera was adsorbed into anti-human IgM-coated wells in plates, and mumps-specific IgM was detected by adding mumps virus hemagglutinin and guinea pig erythrocytes consecutively. Specific IgM-positive sera showed hemadsorption, whereas negative sera showed hemagglutination. All 81 p...

Eighteen paired serum samples submitted for serodiagnosis of current infection showed anomalous antibody results by complement fixation test when tested with a battery of agents (viruses, Mycoplasma pneumoniae, and chlamydia) selected for testing on the basis of the symptoms of the patient. Seventeen serum pairs showed a fourfold or greater rise in titer of antibody to two agents in the battery...