background and purpose: the fusarium species are among the most important fungi in the medical, veterinary and agricultural fields. materials and methods: in the present study, 172 strains of these fungi have been analyzed. the high molecular weight dnas were extracted from 23 reference strains as well as from 149 isolated fusarium species. using the designed nucleotide primers from rdna of fus...

background: dermatophytes are the most common causative agents of superficial mycoses. species identification of these fungi is important from therapeutic and epidemiological point of wive. traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstanc...

background: fusarium species are capable of causing a wide range of crop plants infections as well as uncommon hu­man infections. many species of the genus produce mycotoxins, which are responsible for acute or chronic diseases in ani­mals and humans. identification of fusaria to the species level is necessary for biological, epidemiological, pathologi­cal, and toxicological purposes. in this s...

Calreticulin (CALR) mutations are included in the WHO diagnostic criteria of Myeloproliferative Neoplasm (MPN). Presence CALR mutation, aids their diagnosis by excluding reactive causes. There several molecular techniques to detect mutations; however, these require advanced equipment and technical expertise. An in-house “polymerase chain reaction-restriction fragment length polymorphism (PCR- R...

Dermatofitler, insan ve hayvanlarda keratin içeren dokuları infekte ederek dermatofit infeksiyonuna neden olmaktadırlar. Trichophyton verrucosum sığır dermatofitozis olgularının en yaygın etkenidir. Trichophytosis, bütün dünyada hayvancılık sektöründe önemli ekonomik kayıplara olması yanında zoonoz olmasıyla da sağlığını tehdit etmektedir. Sığırlardan genellikle T. izole edilmektedir. Sığırlar ...

The polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) experiment has the characteristics of low-cost, rapidity, simplicity, convenience, high sensitivity and specificity; thus, many small medium laboratories use it to perform all kinds single nucleotide polymorphisms (SNPs) genotyping works, as a molecular biotechnology for disease-related analysis. However, lack ava...

BACKGROUND Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results. AIM ...

background: in the present study, a pcr-rflp based molecular technique was designed to rapid identification of der­matophytes in clinical specimens. skin scrapings obtained from human cases suspected to dermatophytosis were studied in or­der to identify involved etiological fungi. methods: in this experimental study, the specimens (skin scrapings) of patients referred to mycology department of ...

roots of helianthemum species were collected from various rangeland sites in fars, and other provinces in iran. the partial small subunits of ribosomal dna genes were amplified with the genomic dna extracted from their roots by nested polymerase chain reaction (pcr) using the universal fungal primer pair its1/its4 and specific primer pair ftc/rtc, which was designed based on internal transcribe...