The protein IspH, (E)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate (HMPPP) reductase, is an essential 4Fe–4S cluster-containing protein in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Using a sequence similarity network we found that there are >400 IspH proteins that are about twice as large as most of the IspHs studied to date since their IspH domains are fused to either ...

The protein IspH, (E)-1-hydroxy-2-methyl-but-2-enyl 4-diphosphate (HMPPP) reductase, is an essential 4Fe-4S cluster-containing protein in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Using a sequence similarity network we found that there are >400 IspH proteins that are about twice as large as most of the IspHs studied to date since their IspH domains are fused to either ...

The stability and accuracy of three methods which enforce either a divergence-free velocity field, density invariance, or their combination are tested here through the standard Taylor?Green and spin-down vortex problems. While various approaches to incompressible SPH (ISPH) have been proposed in the past decade, the present paper is restricted to the projection method for the pressure and veloc...

Isoproterenol (ISPH) induced myocardial infarction was confirmed by disturbances in serum and heart tissue marker enzymes such as lactate dehydrogenase (LDH), creatine phospho kinase (CPK), aspartate transaminase (AST) and alanine transaminase (ALT), increased level of lipid peroxidation and histopathological changes in the heart of ISPH administered rats. Pretreatment with mangiferin (10 mg/10...

The nonmevalonate pathway is responsible for isoprenoid production in microbes, including H. pylori, M. tuberculosis and P. falciparum, but is nonexistent in humans, thus providing a desirable route for antibacterial and antimalarial drug discovery. We coordinate a structural study of IspH, a [4Fe-4S] protein responsible for converting HMBPP to IPP and DMAPP in the ultimate step in the nonmeval...

The incompressible Smoothed Particle Hydrodynamics (ISPH) is one of the most popular Lagrangian particle methods for modelling wave-structure interactions. It solves unsteady Navier-Stokes and continuity equations using projection method, in which solving pressure Poisson's equation (PPE) plays a critical role. To discretise Laplacian operator, quadric semi-analytical finite difference interpol...

With current therapies becoming less efficacious due to increased drug resistance, new inhibitors of both bacterial and malarial targets are desperately needed. The recently discovered methylerythritol phosphate (MEP) pathway for isoprenoid synthesis provides novel targets for the development of such drugs. Particular attention has focused on the IspH protein, the final enzyme in the MEP pathwa...

In this paper, the performance of the incompressible SPH (ISPH) method and an improved weakly compressible SPH (IWCSPH) method for free surface incompressible flows are compared and analyzed. In both methods, the Navier–Stokes equations are solved, and no artificial viscosity is used. The ISPH algorithm in this paper is based on the classical SPH projection method with common treatments on soli...

Numerical simulations of fluid-structure interactions in free surface flows were conducted by using an Incompressible smoothed particle hydrodynamics (ISPH) method. In the current ISPH algorithm, a stabilized incompressible SPH method by relaxing the density invariance condition is introduced as Asai et al. (2012). The governing equations are discretized and solved with respect to Lagrangian mo...