background: generally, sewage exposed water could be potentially contaminated with enteroviruses. for this reason, en­terovirus isolation from sewage specimens is one of the most sensitive indicators for virus circulation in the population. we evalu­ated the icc-rt-pcr and cell culture methods for detection of enteroviruses in tehran sewage system. methods: this research utilized 63 specimens p...

We evaluated the use of an integrated cell culture-reverse transcription-PCR (ICC-RT-PCR) procedure coupled with nested PCR to detect human astroviruses, enteroviruses, and adenovirus types 40 and 41 in surface water samples that were collected and evaluated by using the Information Collection Rule (ICR) method. The results obtained with the ICC-RT-PCR-nested PCR method were compared to the res...

The incidence and prognostic relevance of bone marrow (BM) and leukapheresis (PBPC) tumor cell contamination (TCC) in breast cancer patients is still to be circumstantiated. We developed a new comprehensive gene expression panel to study cytokeratins (CK), maspin (MAS) and mammaglobin (MAM) as possible predictors of prognosis. Forty-eight patients undergoing high dose chemotherapy (HDCT) and PB...

Rotaviruses exist widely in water environments and are the major cause to the gastroenteritis in children. To overcome the limitations associated with the current methods for detecting rotaviruses in environmental samples, such as long duration with the traditional cell culture-based plaque assay, inability to detect infectivity with RT-PCR-based molecular methods and lower sensitivity with ELI...

In this study, we studied whether hydrogen sulfide (H(2)S) has an effect on the pacemaker activity of interstitial cells of Cajal (ICC), in the small intestine of mice. The actions of H(2)S on pacemaker activity were investigated using whole-cell patch-clamp technique, intracellular Ca(2+) analysis at 30 and RT-PCR in cultured mouse intestinal ICC. Exogenously applied sodium hydrogen sulfide (N...

Interstitial cells of Cajal (ICC) generate the electrical slow wave required for normal gastrointestinal motility. The ionic conductances expressed in human intestinal ICC are unknown. The aim of this study was to determine expression of a Na+ current in human intestinal ICC and to determine the effects of the Na+ current on the slow wave. Visually identified, freshly dissociated, single ICC we...

Abstract Background Spermatogonial stem cells (SSCs) in the testis are crucial for transferring genetic information to next generation. Successful transplantation of SSCs infertile men is an advanced therapeutic application reproductive biology research. Methods In this experimental research, both vitro and vivo characterization undifferentiated differentiated were performed by morphology—immun...