The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR ...

individual preparation of two human t-cell lymphotropic virus type i (htlv-i) diagnostic gst fused peptides (mta-1 and gd21) is time-consuming and expensive. the aim of this study was to design a novel single chimeric antigen (sca) to obviate separate expression of proteins and reduce the cost of reagent preparation.structural protein fragments, including immunodominant b cell linear epitopes, ...

ABSTRACT: The fluorescence polarization assay (FPA), two variants (V) of the indirect enzyme-linked immunosorbent (I-ELISA) and competitive (C-ELISA) were evaluated in buffaloes to detect antibodies against Brucella spp. V1 I-ELISA identifies them through monoclonal (M23) anti-bovine IgG (I-ELISAM23) V2 ProteinA / G (I-ELISA-A/G). Serum samples 862 (Bubalus bubalis) from Northeast Argentina (NE...

Human T-cell lymphotropic virus type-I (HTLV-I) was the first human retrovirus associated with malignancy.  The prevalence of HTLV-I infection varies significantly in different regions of the world.  In this study, the prevalence of HTLV-I infection among ethnic Jews living in Shiraz, South of Iran, was investigated. 286 blood samples were obtained.  HTLV-1 antibody assay on serum samples was d...

objective: despite sensitive antibody based blood donor screening, infection can be transmitted during window period. therefore sensetive methods bosed on nucleic acid tests (nats) have been considered. the aim of this project was to design a more sensitive method for detection of pcr products and diagnosis of hiv-1/hcv co-infection accurately. materials and methods: after designing specific p...

APPLICATION OF ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE DIAGNOSIS OF BOVINE BRUCELLOSIS IN RIO GRANDE DO SUL, BRAZIL. The results of an indirect ELISA (I-ELISA) and a competitive ELISA (C-ELISA) for detection of antibody to Brucella abortus in cattle were compared with those of conventional serological tests. The sensitivity of I-ELISA using 230 sera from infected animals was 98.6% and the sp...

OBJECTIVE Our aim was to compare the performance of three serological assays in leprosy patients and their household contacts utilising two quantitative ELISA tests using native PGL-I (PGL-1 ELISA), synthetic ND-O-HSA (ND-O-HSA ELISA), and the semi-quantitative lateral flow test (ML Flow). METHODS Comparisons among three immunological assays, PGL-I ELISA, ND-O-HSA ELISA, and ML Flow were perf...

Bluetongue (BT) is an infectious, noncontagious, arthropod-borne viral disease, principally of sheep but many domestic and wild animals are also affected by this disease (Akita et al., 1994). The severity of BT in sheep varies depending on the virus, the breed of sheep and environmental stress. Infection in cattle and goats is usually sub-clinical. Etiological agent of the disease belongs to th...