Objective(s): The aim of this study is to explore the potential role of hypoxia/reoxygenation in necroptosis in cultured rat renal tubular epithelial cell line NRK-52E, and further to investigate its possible mechanisms.Materials and Methods: Cells were cultured under different hypoxia-reoxygenation conditions                        in vitro. MTT assay was used to measure the cell proliferation...

Hypoxic stress, caused by the low cellular oxygen in events of flooding or waterlogging, limits crop productivity many regions world. stress plants is often dynamic and followed a reoxygenation process that returns level to normal. Although metabolic responses hypoxia have been studied plants, less known about recovery processes following removal. To better understand shift from low-oxygen envi...

BackgroundThe volatile anaesthetic sevoflurane protects cardiac tissue from reoxygenation/reperfusion. Mitochondria play an essential role in conditioning. We aimed to investigate how and its primary metabolite hexafluoroisopropanol (HFIP) affect necrosis, apoptosis, reactive oxygen species formation cardiomyocytes upon hypoxia/reoxygenation injury. Moreover, we describe the similarities mode o...

In vivo, left ventricular remodeling after myocardial infarction involves hypertrophy generally attributed to increased cardiac workload. We hypothesized that hypoxia/reoxygenation directly induces cardiomyocyte hypertrophy and studied several participating kinases and transcription factors in isolated cardiomyocytes. Hypoxia for 6 h followed by 42 h reoxygenation induced cardiomyocyte hypertro...

BACKGROUND Acute hypoxia results in increased pulmonary vascular resistance. Despite reoxygenation, pulmonary vascular resistance remains elevated and pulmonary function is altered. Endothelin-1 might contribute to hypoxia-reoxygenation-induced pulmonary hypertension and to reoxygenation injury by stimulating leukocytes. This study was carried out using an established model of hypoxia and reoxy...

CONTEXT Exposure of hepatocytes to pathological conditions in a microenvironment of hypoxia and reoxygenation is very frequent in hepatic diseases. Several substances present perspectives for cytoprotective action on hepatocyte submitted to reoxygenation after hypoxia and simple hypoxia. OBJECTIVE We research therapeutic options for hepatocytes submitted to hypoxia and hypoxia + reoxygenation...

To investigate factors contributing to reperfusion and reoxygenation myocardial injury, we exposed layers of cultured chick ventricular myocytes to severe hypoxia for up to 3 hours in the presence of 20 mM 2-deoxyglucose, zero glucose, and 5 mM pyruvate, and then exposed the myocytes to reoxygenation. Lactate dehydrogenase (LDH) release was moderately increased during 3 hours of hypoxia but was...

The fluctuations of dissolved oxygen (DO) often lead to hypoxia in aquaculture, which has a huge adverse impact on fish. This study mainly investigated the effects long-term oxidative stress, immune response, and cell apoptosis liver golden pompano ( Trachinotus blochii ), is not tolerant hypoxia. So we conducted 14 day low stress experiment with DO 3.0 ± 0.2 mg/L, then restore normal levels co...