background and aim(s): this in vitro study compared the effects of mineral trioxide aggregate (mta), calcium enriched mixture (cem) cement, biodentine (bd) and octacalcium phosphate (ocp) on the viability of human gingival fibroblasts (hgfs). material and methods: after completion of the setting time of understudy materials, fibroblasts were placed in 24-well insert plates and 1mg of the unders...

Background  Recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (LLLT). The aim of this study was to determine whether LLLT could induce a bio-stimulatory effects on human gingival fibroblasts (HGF3-PI 53). Therefore, the effect of laser irradiation on human gingival cell proliferation and collagen type I gene ...

BACKGROUND We previously demonstrated that 25-hydroxyvitamin D(3) concentrations in gingival crevicular fluid are 300 times higher than those in the plasma of patients with aggressive periodontitis. Here we explored whether 25-hydroxyvitamin D(3) can be synthesized by periodontal soft tissue cells. We also investigated which of the two main kinds of hydroxylases, CYP27A1 and CYP2R1, is the key ...

Bahammam M, Black SA, Jr, Sume SS, Assaggaf MA, Faibish M, Trackman PC. Requirement for active glycogen synthase kinase-3 in TGF1 upregulation of connective tissue growth factor (CCN2/CTGF) levels in human gingival fibroblasts. Am J Physiol Cell Physiol 305: C581–C590, 2013. First published July 3, 2013; doi:10.1152/ajpcell.00032.2013.—Connective tissue growth factor (CCN2/CTGF) mediates transf...

Objective: Kaempferol is a natural flavonol found in many dietary fruits, vegetables, and medicinal herbs. It reportedly has multiple beneficial properties as analyzed in vivo and in vitro. The objective of this study was to determine cytoprotective, anti-inflammatory, and wound healing characteristics of kaempferol in cultured human gingival fibroblasts. Materials & Methods: To determine cytot...

introduction: the aim of this study was to compare the cytotoxicity of proroot mta (pmta), root mta (rmta) and portland cement (pc) on human gingival fibroblasts (hgfs). materials and methods: the extracts of the test materials were placed close to human gingival fibroblasts (hgfs) within 96-well plates. cellular viability was assessed using mtt assay in different intervals (freshly mixed, 4, 2...

background  recent investigations show that both proliferation and secretion of macromolecules by cells can be regulated by low level laser therapy (lllt). the aim of this study was to determine whether lllt could induce a bio-stimulatory effects on human gingival fibroblasts (hgf3-pi 53). therefore, the effect of laser irradiation on human gingival cell proliferation and collagen type i gene e...

Since human gingival fibroblasts are the major cells in periodontal tissues, we hypothesized that gingival fibroblasts are endowed with receptors for bacterial components, which induce innate immune responses against invading bacteria. We found clear mRNA expression of Toll-like receptors (TLR)1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, MD-2, MyD88, NOD1, and NOD2 in gingival fibroblasts...

BACKGROUND We previously demonstrated that 25-hydroxyvitamin D(3), the precursor of 1α,25-dihydroxyvitamin D(3), is abundant around periodontal soft tissues. Here we investigate whether 25-hydroxyvitamin D(3) is converted to 1α,25-dihydroxyvitamin D(3) in periodontal soft tissue cells and explore the possibility of an autocrine/paracrine function of 1α,25-dihydroxyvitamin D(3) in periodontal so...