a pair of degenerate primers, gmpf1 and gmpr1, was designed on the basis of alignment of previously reported grapevine fanleaf virus (gflv) movement protein (mp) nucleotide sequences from iran and other parts of the world. cdna was synthesized by the use of oligo d(t)18 from total rna extraction from each diseased grapevine leaf sample and subjected to polymerase chain reaction (pcr) with the d...
