Cytoplasmic dynein transports membranous cargoes along microtubules, but the mechanism of dynein-cargo interaction is unclear. From a genetic screen, we identified a homologue of human Hook proteins, HookA, as a factor required for dynein-mediated early endosome movement in the filamentous fungus Aspergillus nidulans. HookA contains a putative N-terminal microtubule-binding domain followed by c...

The minus end-directed microtubule motor cytoplasmic dynein transports various cellular cargoes, including early endosomes, but how dynein binds to its cargo remains unclear. Recently fungal Hook homologues were found to link dynein to early endosomes for their transport. Here we identified FhipA in Aspergillus nidulans as a key player for HookA (A. nidulans Hook) function via a genome-wide scr...

Early endosomes are transported bidirectionally by cytoplasmic dynein and kinesin-3, but how the movements are regulated in vivo remains unclear. Here our forward genetic study led to the discovery of VezA, a vezatin-like protein in Aspergillus nidulans, as a factor critical for early endosome distribution. Loss of vezA causes an abnormal accumulation of early endosomes at the hyphal tip, where...

M any organelles move through the cytoplasm along micro-tubules, pulled back and forth by microtubule-based motor proteins. Early endosomes, for example, are transported by the minus end–directed motor dynein and the plus end–directed motor kinesin-3, which are thought to engage in a " tug of war " with each other as they attempt to haul their cargo in opposite directions. Zhang et al. (1) and ...

abstract introduction: smoking is one of the sanitary problems in world today. increasing smoking of cigarette and other tobacco products has concerned health managers and community responsible. in recent decades, smoking has increased specially in developing countries and the smokers are adding every year. the objective of this study is determination of smoking prevalence and its distribution ...

BACKGROUND & OBJECTIVES Of the various biochemical markers used to validate the smoking status of a person, nicotine and continine are considered as good markers for both active and passive smoking. In the present study an attempt was made to estimate urinary levels of nicotine and cotinine in healthy individuals from north India using different types of tobacco to identify and validate the smo...

background:globally, smoking is one of the main causes of morbidity and mortality. it is also an important social determinant of health and the largest contributor to health inequalities. while several prevalence studies are conducted on special groups such as physicians, less such studies have been focused on deprived areas (areas with lacking adequate food, shelter, education, etc). the aim o...

CONTEXT The global tobacco epidemic may kill 10 million people annually in the next 20-30 years, with 70% of these deaths occurring in developing countries. Current research, treatment, and policy efforts focus on cigarettes, while many people in developing regions (Asia, Indian subcontinent, Eastern Mediterranean) smoke tobacco using waterpipes. Waterpipes are increasing in popularity, and mor...

INTRODUCTION Hookah smoking is becoming increasingly popular among young adults and is often perceived as less harmful than cigarette use. Prior studies show that it is common for youth and young adults to network about substance use behaviors on social media. Social media messages about hookah could influence its use among young people. We explored normalization or discouragement of hookah smo...