Alu elements are retrotransposons that frequently form new exons during primate evolution. Here, we assess the interplay of splicing repression by hnRNPC and nonsense-mediated mRNA decay (NMD) in the quality control and evolution of new Alu-exons. We identify 3100 new Alu-exons and show that NMD more efficiently recognises transcripts with Alu-exons compared to other exons with premature termin...

Alternative polyadenylation (APA) is a general mechanism of transcript diversification in mammals, which has been recently linked to proliferative states and cancer. Different 3' untranslated region (3' UTR) isoforms interact with different RNA-binding proteins (RBPs), which modify the stability, translation, and subcellular localization of the corresponding transcripts. Although the heterogene...

Coordinate, Dominant-Negative Control of Estradiol-Directed Gene Transactivation by an Intracellular Estrogen Binding Protein (Heat shock protein 27; Hsp27) and an Estrogen Response Element Binding Protein (hnRNPC-like Protein) John S. Adams1, Hong S. Chen2, Martin S. Hewison2 1Orthopaedic Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA; 2Burns and Allen Research Institute, Ce...

Urokinase-type plasminogen activator (uPA) and its receptor (uPAR) play a major role in several physiological processes such as cell migration, proliferation, morphogenesis, and regulation of gene expression. Many of the biological activities of uPA depend on its association with uPAR. uPAR expression and its induction by uPA are regulated at the posttranscriptional level. Inhibition of protein...

RNA-protein binding is critical to gene regulation, controlling fundamental processes including splicing, translation, localization and stability, and aberrant RNA-protein interactions are known to play a role in a wide variety of diseases. However, molecular understanding of RNA-protein interactions remains limited; in particular, identification of RNA motifs that bind proteins has long been c...

Abstract Background We aimed to construct and validate the esophageal squamous cell carcinoma (ESCC)-related m6A regulators by means of machine leaning. Methods used ESCC RNA-seq data 66 pairs from West China Hospital Sichuan University transcriptome extracted The Cancer Genome Atlas (TCGA)-ESCA database find out ESCC-related regulators, during which, two learning approaches: RF (Random Forest)...

Background Increasing evidence elucidated N6-methyladenosine (m6A) dysregulation participated in regulating RNA maturation, stability, and translation. This study aimed to demystify the crosstalk between m6A regulators immune microenvironment, providing a potential therapeutic target for patients with hepatocellular carcinoma (HCC). Methods Totals of 371 HCC 50 normal were included this study. ...