Amplified fragment length polymorphism (AFLP) was used to investigate polymorphism among 43 Hirsutella thompsonii isolates (33 from Thailand) obtained from various mite species. The outgroups were an unidentified Hirsutella isolate along with Hirsutella nodulosa and Hirsutella kirchneri. Phylogenetic analyses of the AFLP data showed significant variation among isolates and the existence of thre...

Penetration of cabbage roots by Heterodera schachtii was suppressed 50-77% in loamy sand naturally infested with the nematophagous fungus Hirsutella rhossiliensis. When Heterodera schachtii was incubated in the suppressive soil without plants for 2 days, 40-63% of the juveniles had Hirsutella rhossiliensis spores adhering to their cuticles. Of those with spores, 82-92% were infected. Infected n...

Hirsutella (Ophiocordycipitaceae: Hypocreales) is a genus of insect, mite, and nematode pathogens with an asexual morph, which generally produce a mucilaginous cluster of one or several conidia on phialides that are basally subulate and taper to a fine neck. The generic name Hirsutella has been proposed for suppression in favour of Ophiocordyceps as a consequence of the ending of dual nomenclat...

The ability of Hirsutella rhossiliensis to colonize various substrates in sterile and nonsterile soil was measured. Hirsutella rhossiliensis was recovered from 67% and 77% of living, inoculated Criconemella xenoplax incubated in sterile and nonsterile soil, respectively. In contrast, the fungus was recovered from 100% and 18% of heat-killed, inoculated nematodes incubated on sterile and nonster...

The impact of the nematode-parasitic fungus Hirsutella rhossiliensis on the effectiveness of Steinernema carpocapsae, S. glaseri, and Heterorhabditis bacteriophora against Galleria mellonella larvae was assessed in the laboratory. The presence of Hirsutella conidia on the third-stage (J3) cuticle of S. carpocapsae and H. bacteriophora interfered with infection of insect larvae. Conidia on the J...

Biological control by beneficial microorganisms is known to significantly reduce the effect of pests on crops yield. Among biocontrol strategies use entomopathogenic fungi such as Hirsutella citriformis, which has been applied infect and kill hemipteran insect pests, including Diaphorina citri Kuwayama (Hemiptera: Liviidae) Bactericera cockerelli Sulc. Triozidae). These biological agents are in...

Eleven fungal isolates were tested in agar dishes for pathogenicity to Pratylenchus penetrans. Of the fungi that produce adhesive conidia, Hirsutella rhossiliensis was a virulent pathogen; Verticillium balanoides, Drechmeria coniospora, and Nematoctonus sp. were weak or nonpathogens. The trapping fungi, Arthrobotrys dactyloides, A. oligospora, Monacrosporium dlipsosporum, and M. cionopagum, kil...

Eight new species of the genus Coleophora Hübner, 1822 are described: Coleophora kenyaensis sp. nov., C. vansoni sp. nov., C. elegans sp. nov., C. namaqua sp. nov., C. kamiesella sp. nov., C. kunenensis sp. nov., C. creola sp. nov., and C. hirsutella sp. nov. The genitalia of three previously described species are described and illustrated for the first time: Coleophora niphocrossa Meyrick, 192...

A spore assay was developed to measure the relative density of spores of the nematophagous fungus Hirsutella rhossiliensis in soil. Orchard soil containing H. rhossiliensis-parasitized Criconemella xenoplax was placed in vials and incubated for 0-120 days before the addition of probe nematodes, Heterorhabditis heliothidis juveniles. After 18 hours, H. heliothidis were extracted from the soil an...