نتایج جستجو برای: High-resolution melt analysis

تعداد نتایج: 4541240  

Background & Objective:  Vibrio cholerae is a natural inhabitant of the environment and causes severe diarrhea ailments (cholera) that affects thousands of people each year worldwide. The most important virulence factors of this pathogen are cholera toxin (cholera toxin CT) and Type IV...

Ali Reza Vahdati Malike Sharif, Mojtaba Hashemi Reza Mirnejad, Vahhab Piranfar,

Objective(s): Rapid and accurate detection of Brucella abortus and Brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. This study reveals a new method in detection of clinical samples of brucellosis using real-time PCR and high-resolution melt (HRM) curve analysis. Materials and Methods: 160 brucellosis suspicious samples with more tha...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه مازندران - دانشکده علوم انسانی و اجتماعی 1388

abstract the present study was conducted to examine the effect of proficiency on students interaction while carrying out the grammar-based task(s) in collaboration. in particular, the study examines whether the level of proficiency affects the learners’ focus of attention to grammatical and lexical features. the study takes a further step and examines whether there is any difference in the use...

2010
Sean Taylor Rachel Scott Richard Kurtz Carl Fisher Viresh Patel

Introduction Classifying and understanding genetic variation between populations and individuals is an important aim in the field of genomics. Many common diseases (diabetes, cancer, osteoporosis, etc.) and clinically relevant phenotypic traits are elicited from the complex interaction between a subset of multiple gene products and environmental factors. The unique genetic profile of an individ...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد علوم دارویی - پژوهشکده ابن سینا 1392

روش های متنوعی برای شناسایی جهش ها ابداع شده است که هریک از آنها دارای مزایا و معایب خاص خود هستند. یکی از روش هایی که اخیراً ابداع شده و در مطالعات مختلف به کارآیی بالای آن اشاره شده است روش high resolution melting analysis یا hrm است. در مطالعه حاضر برای اولین بار در ایران به منظور شناسایی جهش ها تمامی اگزون های ژن rb1 با استفاده از روش تعیین توالی بررسی شده است. در ادامه به منظور بررسی حساسیت...

2010
Carl Fisher Ray Meng Francisco Bizouarn Rachel Scott

<0.02°C temperature increments on a system with <0.02°C uniformity variability (Tesoriero 2008). However, successful discrimination of sequence variants, including the most difficult-to-detect class IV SNPs, is not solely dependent on the temperature increments from which melt data are collected. Several factors influence DNA melting behavior, including reaction mix composition, amplicon size, ...

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