Background & Objective:  Vibrio cholerae is a natural inhabitant of the environment and causes severe diarrhea ailments (cholera) that affects thousands of people each year worldwide. The most important virulence factors of this pathogen are cholera toxin (cholera toxin CT) and Type IV...

Objective(s): Rapid and accurate detection of Brucella abortus and Brucella melitensis from clinical samples is so important because antibiotic treatment has major side effects. This study reveals a new method in detection of clinical samples of brucellosis using real-time PCR and high-resolution melt (HRM) curve analysis. Materials and Methods: 160 brucellosis suspicious samples with more tha...

Introduction Classifying and understanding genetic variation between populations and individuals is an important aim in the field of genomics. Many common diseases (diabetes, cancer, osteoporosis, etc.) and clinically relevant phenotypic traits are elicited from the complex interaction between a subset of multiple gene products and environmental factors. The unique genetic profile of an individ...

<0.02°C temperature increments on a system with <0.02°C uniformity variability (Tesoriero 2008). However, successful discrimination of sequence variants, including the most difficult-to-detect class IV SNPs, is not solely dependent on the temperature increments from which melt data are collected. Several factors influence DNA melting behavior, including reaction mix composition, amplicon size, ...