the efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. conclusions were drawn regarding selection of solid phase according to pore size, rigidity, ph stability, chemistry of derivation and activation, and gross concentration of immobilized ligand required fo...

background: a simple and sensitive high performance liquid chromatography-electrospray ionization mass spectrometry method has been evaluated for the assignment of clonidine hydrochloride in human plasma. methods: the mobile phase composed of acetonitrile-water 60:40 (v/v) and 0.2% formic acid 20 µl of sample was chromatographically analyzed using a repacked zorbax-xdb-ods c18 column (2.1 mmx30...

background aflatoxins are one of the most potent toxic substances that occur naturally. nowadays extensive attention has been taken to their existence in food and environment, as there is the possibility of harm to humans following chronic exposure to extremely low levels via food chain. aflatoxin m1 (afm1) is a hepatic carcinogenic metabolite found in the milk of lactating animals fed with con...

separation of ten sedative- hypnotic drugs was performed by rp-hplc using mixed micellar mobile phase. effect of temperature, type and amount of organic modifier in mobile phase on efficiency (n) and asymmetry factor (b/a) showed that, the appropriate conditions for a good separation were 35°c and 7% (v/v) butanol in mobile phase.  variations of selectivity factor versus butanol concentration, ...

conclusions this method could be feasibly used for purification of large quantities of allicin from garlic extract. results semi-preparative hplc yielded a distinct peak with a retention time of 12.28 minutes. the purity (≥ 95%) and biological activity (p > 0.05) of purified allicin were comparable to the standard allicin. purified allicin produced same peak areas compared to the standard allic...

in the present study, a comprehensive and systematic strategy was described to evaluate the performance of several three-way calibration methods on a bio-analytical problem. parallel factor analysis (parafac), alternating trilinear decomposition (atld), self-weighted alternating trilinear decomposition (swatld), alternating penalty trilinear decomposition (aptld) and unfolded partial least squa...

a stability indicating high-performance liquid chromatography (hplc) method wasvalidated and used to study the degradation of cetirizine dihydrochloride in acidic and oxidativeconditions. the separation was carried out on a symmetry c18 column and a mixture of 50 mmkh2po4 and acetonitrile (60:40 v/v, ph = 3.5) was used as the mobile phase. the method waslinear over the range of 1-20 μg/ml of ce...

background: cattle can be considered as an important source for herbicides through nutrition. therefore, herbicide residue in animal products is a potential human exposure to herbicides causing public health problems in human life. triazines are a group of herbicides primarily used to control broadleaf weeds in corn and other feed ingredients and are considered as possible human carcinogens. to...

background & aim: according to the increase in diseases such as diabetes and obesity, using of saccharose alternatives for producing of low-calorie and sugar-free alternatives, are taken into consideration of researchers and industrialists. among them, steviosidewith the 300 times more sweetness than saccharose has special place, due to is a natural. the aim of this study is optimizing of stevi...