conclusions the study results showed that stimulation of the american cockroach’s immunity system lead to production of antibacterial proteins and peptides which had inhibitory effect on the bacteria, depending on the bacterial strains and their sensitivity. likely this feature of insects can be used as therapeutic strategies to produce natural antimicrobial compounds against the pathogenic bac...

background: although cockroaches are mechanical vector of many pathogens, still biological transmission of pathogens via these insects is controversial. more covenant immune defense in the hemolymph of cockroaches may take this ad­vantage to block any adaptation of pathogens regarding to biological transmission. lectin molecules in hemolymph of in­sects play as mediators of nonself recognition,...

Bio-analysis of insects is increasingly dependent on highly sensitive methods that require high quality biological material, such as hemolymph. However, it is difficult to collect fresh and uncontaminated hemolymph from adult bees since they are very active and have the potential to sting, and because hemolymph is rapidly melanized. Here we aimed to develop and test a quick and easy method for ...

Insect feeding behavior is regulated by many intrinsic factors, including hemolymph nutrient levels. Adipokinetic hormone (AKH) is a peptide factor that modulates hemolymph nutrient levels and regulates the nutritional state of insects by triggering the transfer of lipids into the hemolymph. We recently demonstrated that RNA interference (RNAi)-mediated knockdown of the AKH receptor (AKHR) redu...

Purpose: selection of anticoagulants based on EDTA-Na 2 and methods their use for working with the hemolymph Australian red claw crayfish (C. quadricarinatus) Materials methods. For study, different-sized males females australian red-clawed (Cherax quadricarinatus (Von Martens, 1868)) were used. was used as an anticoagulant, concentrationof which 4%. A ml syringe a 23G needle removal pre-washed...

High density lipophorin (HDLp) from the hemolymph of the German cockroach, Blattella germanica (L.) (Family Blattellidae), has an apparent molecular weight of 670kDa, with an isoelectric point of 7.0 and a density of 1.109g/ml. It is composed of two subunits, apolipoprotein-I (212kDa) and apolipoprotein-II (80kDa), and consists of 51.4% lipid, 46.2% protein and 2.4% carbohydrate. Hydrocarbons c...

The wings of insects are composed of membranes supported by interconnected veins. Within these veins are epithelial cells, nerves and tracheae, and their maintenance requires the flow of hemolymph. For this purpose, insects employ accessory pulsatile organs (auxiliary hearts) that circulate hemolymph throughout the wings. Here, we used correlative approaches to determine the functional mechanic...

schistosomiasis is the second most common parasitic cause of death, after malaria. biomphalaria glabrata is a fresh water snail with medical importance since it is the intermediate host of schistosoma mansoni, an agent of schistosomiasis. the internal defense system of b. glabrata snails is mostly represented by circulating elements of the hemolymph, hemocytes, which are important factors in fi...

The handling of Na(+) and K(+) loads was investigated in isolated Malpighian tubules and in whole mosquitoes of Aedes aegypti. Isolated Malpighian tubules bathed in Na(+)-rich Ringer solution secreted Na(+)-rich fluid, and tubules bathed in K(+)-rich Ringer solution secreted K(+)-rich fluid. Upon Na(+) loading the hemolymph, the mosquito removed 77% the injected Na(+) within the next 30 min. Th...