Light and scanning electron micrographs of Opisthorchis viverrini, Haplorchis taichui, H. pumilio, A Phaneropsolus bonnei, and Prosthodendrium molenkampi eggs were studied. Under light microscopy. O. viverrini eggs had rough eggshells and prominent shoulders. H. taichui, H. pumilio were similar in shape and had smooth eggshells and prominent shoulders. H. pumilio eggs were bigger than H. taichu...

This study aimed to investigate Haplorchis taichui metacercarial infection in fish collected from the Chom Thong and Mae Taeng districts, Chiang Mai Province during November 2001 to October 2002. A total 617 cyprinoid fish of 15 species were randomly collected and examined for H. taichui metacercariae. All the species of fish were found to be infected with H. taichui. The infection rates were 9...

We report an exceptionally high rate of infection by Haplorchis taichui (Nishigori, 1924) in human populations on Mindanao Island, southern Philippines. This intestinal fluke is seldom encountered, and this is the first report of high prevalence of infection (36%) in humans by H. taichui in the Philippines. The likely source of haplorchine infection has been linked to consumption of raw or unde...

Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and seque...

In this study, we found that Haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in Saravane and Champasak province, Lao PDR. Fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (Mekong river) districts and were examined by the Kato-Katz fecal smear technique. The overall helminth egg positive ...

The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra...

The infection status of fishborne zoonotic trematode (FZT) metacercariae was investigated in fishes from 2 localities of Lao PDR. Total 157 freshwater fishes (17 species) were collected in local markets of Vientiane Municipality and Champasak Province in December 2010 and July 2011, and each fish was examined by the artificial digestion method. Total 6 species of FZT metacercariae, i.e., Opisth...

Abstract. A seasonal investigation of the occurrence of metacercariae in cyprinoid fish was conducted at the Kwae Noi Bamroongdan Dam, Phitsanulok Province, northern Thailand during December 2008-August 2009. A total 361 cyprinoid fish from 10 species were examined by artificial digestion method evaluating for metacercariae. The metacercariae determined were Haplorchis taichui, Haplorchis pumil...

The development of Haplorchis taichui was studied in sixteen mice, Mus musculus. Metacercarial cysts of H. taichui were obtained from the freshwater fish, Thynnichthys thynnoides, collected in Chiang Mai Province, north Thailand. Approximately 200 active metacercariae were orally introduced into each mouse. Two mice were randomly sacrificed and necropsied daily from day 2-9 postinfection (pi). ...

Small liver and minute intestinal flukes are highly prevalent in Southeast Asia, and in mixed infections, their eggs are difficult to differentiate morphologically in fecal samples. PCR assays targeting the ITS regions in ribosomal DNA were designed to identify and differentiate species. The PCR amplicons of Opisthorchis viverrini, Clonorchis sinensis, Haplorchis pumilio, and Haplorchis taichui...