an oil adjuvant vaccine (oav) was prepared from a local strain of pasteurella multocida. strain 6:b was cultured and inactivated by formalin. bacterial pellet was prepared by centrifugation and subsequently adjuvanted by montannide oil isa-70. a dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by im route. animals were bled before and at 24, 90, 150, and...

haemorrhagic septicaemia (hs) vaccine which is prepared in razi institute is used in endemic areas ofiran. aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. post-vaccinal shock reactionswere the main complaint after use of this vaccine. in a previous study, we could improve the vaccine byalum-precipitation pasteurella multocida cells and removing the liquid phase. in this ...

haemorrhagic septicaemia (hs) is a fatal systemic disease of cattle and buffaloes. some control isachieved with administration of alum-precipitated or oil-adjuvanted killed whole-cell vaccines injectedsubcutaneously. these vaccines, however, provide only short-term immunity and for effective use, theyshould be administered annually. we constructed an aroa attenuated derivative of a pasteurella ...

Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas ofIran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactionswere the main complaint after use of this vaccine. In a previous study, we could improve the vaccine byalum-precipitation Pasteurella multocida cells and removing the liquid phase. In this ...

background and objectives : haemorrhagic septicaemia (hs), caused by pasteurella multocida, is the most important bacterial disease of cattle and buffaloes in india. oil adjuvant vaccine (oav) is the most potent vaccine available for the control of hs. the study aims to evaluate the effect of alum co-adjuvantation of oav on emulsion stability and immune response. materials and methods : two dif...

Twelve apparently healthy and recently parturiated Murrah buffaloes, vaccinated with Foot-and-mouth disease (FMD) + Haemorrhagic septicaemia (HS) combined vaccine at an organized farm along their newborn calves were inducted for detection of antibodies in serum colostrum against FMD virus (FMDV) serotypes O, A Asia-1, Pasteurella multocida using enzyme linked immunosorbent assay. The born to da...

0

Aim: The present study was carried out in 100 cattle to assess the antibody response to Haemorrhagic Septicaemia alum precipitated vaccine by Microtiter Agglutination Test (MAT), Indirect Haemaaglutination Assay (IHA) and Monoclonal Antibody based Indirect Enzyme Linked Immunosorbent Assay (ELISA). Materials and Methods: One hundred cattle from a local gaushala of Ludhiana were vaccinated with ...

Pasteurella multocida (P. multocida) is an important pathogen of various domestic animals. The outer membrane proteins (OMPs) play a major role in pathogenesis and immunogenicity of P. multocida. The aim of the study was to develop indirect enzyme linked immuno sorbant assay (ELISA) based on OMPs to ascertain the antibody titers in animals post-infection or to gauge the potency of vaccine. The ...