نتایج جستجو برای: HTS (High-Throughput Screening)

تعداد نتایج: 2257310  

Journal: :biomacromolecular journal 2015
fatemeh ahmadi reza sajedi atiyeh mahdavi mehdi zeinoddini majid taghdir

photoproteins are excellent reporter systems because they don’t have virtually background signal. aequorin is the most well-known photoprotein. three improved engineered photoproteins photina, i-photina and c-photina, were also recently developed and optimized for generation of ca2+ mobilization assays precisely. the total light emission is greater than aequorin and their reaction kinetics is a...

ژورنال: :طب جنوب 0
فاطمه احمدی fatemeh ahmadi department of biochemistry, faculty of biological sciences, tarbiat modares university, tehran, iranگروه بیوشیمی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران رضا حسن ساجدی reza h. sajedi department of biochemistry, faculty of biological sciences, tarbiat modares university, tehran, iranگروه بیوشیمی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران مهدی زین الدینی mehdi zeinoddini department of bioscience and biotechnology, mallek ashtar university of technology, tehran, iranپژوهشکده علوم و فناوری زیستی، دانشگاه صنعتی مالک اشتر، تهران، ایران

زمینه: امروزه استفاده از فتوپروتئین ها به واسطه سیگنال کم پس زمینه ای جایگاهی ویژه یافته است. از بین فتوپروتئین های رایج اکثر مطالعات روی فتوپروتئین اکورین متمرکز شده است. اما به دلیل پایین بودن شدت سیگنال و نیز بالا بودن سرعت خاموشی آن در سنجش های hts (high-throughput screening) چندان کارآمد نمی باشد. در این راستا محققین در سال 2004 سه فتوپروتئین ارتقاء یافته به منظور تشخیص مقادیر ناچیز کلسیم ...

2017
Andrew R Collins Balasubramanyam Annangi Laura Rubio Ricard Marcos Marco Dorn Carolin Merker Irina Estrela-Lopis Mihaela Roxana Cimpan Mohamed Ibrahim Emil Cimpan Melanie Ostermann Alexander Sauter Naouale El Yamani Sergey Shaposhnikov Sylvie Chevillard Vincent Paget Romain Grall Jozo Delic Felipe Goñi- de-Cerio Blanca Suarez-Merino Valérie Fessard Kevin N Hogeveen Lise Maria Fjellsbø Elise Runden Pran Tana Brzicova Jan Topinka Maria João Silva P E Leite A R Ribeiro J M Granjeiro Roland Grafström Adriele Prina-Mello Maria Dusinska

With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety-preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read-across approaches. The adoption of high throughput screen...

Journal: :Nature chemical biology 2007
James Inglese Caroline E Shamu R Kiplin Guy

Publications reporting results of small-molecule screens are becoming more common as academic researchers increasingly make use of high-throughput screening (HTS) facilities. However, no standards have been formally established for reporting small-molecule screening data, and often key information important for the evaluation and interpretation of results is omitted in published HTS protocols. ...

2013
Douglas Jay Klein Hongyao Zhu Jacquelyn Klug-McLeod Gregory A. Bakken

A heuristic algorithm was developed to maximize compound diversity within a subset of screening plates used in high throughput screening (HTS) to initiate the drug discovery process. The approach overcame the challenge of combinatorial explosion for selecting plate subsets with maximum compound diversity. The method yielded novel forms of plate-based diversity subsets (PBDS) for HTS screening i...

Journal: :Journal of computer-aided molecular design 2013
Yufeng J. Tseng Eric J. Martin Cristian Bologa Anang A. Shelat

The "Cheminformatics aspects of high throughput screening (HTS): from robots to models" symposium was part of the computers in chemistry technical program at the American Chemical Society National Meeting in Denver, Colorado during the fall of 2011. This symposium brought together researchers from high throughput screening centers and molecular modelers from academia and industry to discuss the...

2017
Gergely Kosa Volha Shapaval Achim Kohler Boris Zimmermann

BACKGROUND Analyses of substrate and metabolites are often bottleneck activities in high-throughput screening of microbial bioprocesses. We have assessed Fourier transform infrared spectroscopy (FTIR), in combination with high throughput micro-bioreactors and multivariate statistical analyses, for analysis of metabolites in high-throughput screening of microbial bioprocesses. In our previous st...

2012
Ru Zang Ding Li I-Ching Tang Jufang Wang Shang-Tian Yang

Drug screening is a long and costly process confronted with low productivity and challenges in using animals, which limit the discovery of new drugs. To improve drug screening efficacy and minimize animal testing, recent efforts have been dedicated to developing cell-based high throughput screening (HTS) platforms that can provide more relevant in vivo biological information than biochemical as...

Journal: :Briefings in bioinformatics 2015
Iurie Caraus Abdulaziz A. Alsuwailem Robert Nadon Vladimir Makarenkov

Significant efforts have been made recently to improve data throughput and data quality in screening technologies related to drug design. The modern pharmaceutical industry relies heavily on high-throughput screening (HTS) and high-content screening (HCS) technologies, which include small molecule, complementary DNA (cDNA) and RNA interference (RNAi) types of screening. Data generated by these ...

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