A novel, simple, and versatile amperometric sensing platform was developed based on HRP-mimicking DNAzyme-catalyzed template-guided deposition of polyaniline (PANI). Pb(2+) was chosen as a model to demonstrate the proof-of-concept of our approach. The Pb(2+) aptamer was first self-assembled on the electrode surface. Upon addition of Pb(2+), Pb(2+) bound to its aptamer to form G-quadruplex (G4)....

For the first time, hemin/G-quadruplex was employed to simultaneously serve as NADH oxidase and an HRP-mimicking DNAzyme for constructing a simple and sensitive pseudobienzyme-amplifying electrochemical aptasensor for thrombin detection.

The binding behavior of a competitive inhibitor, N-phenylbenzhydroxamic acid (BHA) against horseradish peroxidase (HRP) was studied in order to understand and predict the interaction mechanism of hydrogen donors with the enzyme. The dissociation constants of the complexes of HRP-BHA, HRP-donor and HRP-BHA-azide were estimated at specified conditions by difference spectroscopy. The binding s...

In the present study, we fabricated an ultrasensitive sandwich-type electrochemical aptasensor for thrombin (TB) based on a triplex signal amplification strategy. The hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) as well as blocking reagent-horseradish peroxidase (HRP) and iron telluride nanorods (FeTe NRs) could simultaneously amplify the electrochemical signal of t...

the binding behavior of a competitive inhibitor, n-phenylbenzhydroxamic acid (bha) against horseradish peroxidase (hrp) was studied in order to understand and predict the interaction mechanism of hydrogen donors with the enzyme. the dissociation constants of the complexes of hrp-bha, hrp-donor and hrp-bha-azide were estimated at specified conditions by difference spectroscopy. the binding site ...

Herein, we report preparation of magnetic hybrid nanoflower (MhNF) formed horseradish peroxidase (HRP), copper ions (Cu2+) and iron oxide nanoparticles (Fe3O4 NPs) with greatly enhanced activity, stability, reusability applicability for use in removal phenol from aqueous solution. In this study, pre-synthesized small sized Fe3O4 NPs were immobilized to synthesized HRP-Cu2+ (hNFs). The MhNFs cha...

As an emerging nonthermal technology, cold plasma has been used to inactivate endogenous enzymes that are responsible for enzymatic browning reaction of fruits and vegetables. This study aimed investigate the inactivation effect dielectric barrier discharge (DBD) on horseradish peroxidase (HRP), a typical plant peroxidase. The results showed DBD caused HRP in time-and power-dependent manner. ac...

Hepatoma-derived growth factor-related protein-3 (Hdgfrp3 or HRP-3) was recently reported as a neurotrophic factor and is upregulated in hepatocellular carcinoma to promote cancer cell survival. Here we identified HRP-3 as a new endothelial ligand and characterized its in vitro and in vivo functional roles and molecular signaling. We combined open reading frame phage display with multi-round in...

In this study, the structural characters, antioxidant activities and bile acid-binding ability of sea buckthorn polysaccharides (HRPs) obtained by commonly used hot water (HRP-W), pressurized (HRP-H), ultrasonic (HRP-U), acid (HRP-C) alkali (HRP-A) assisted extraction methods were investigated. The results demonstrated that had significant effects on yield, monosaccharide composition, molecular...

This study describes the fabrication techniques for two forms of magnetic DNA nanotubes (MDNTs) and their applications as platforms for developing colorimetric assays. The first form of MDNTs was DNTs filled-up with magnetic nanoparticles (MNPs) and the second one was DNTs arayed with MNPs on their extrior surfaces. Then the both forms of MDNTs were employed as platforms for attaching a specifi...