Snake venoms comprise a highly complex mixture of proteins, and there is also high interspecific intraspecific variability in their composition, even the same region. Our aim was to compare composition Bothrocophias myersi, Crotalus durissus Bothrops asper, snakes from Andean region Colombia by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). The were given research group under a...

IB-16385A Abstract Capillary electrophoresis sodium dodecyl sulfate (CE-SDS), is the modern equivalent of the slabgel sizing technique SDS-PAGE. Common uses of SDS-PAGE include monitoring of manufacturing consistency and apparent molecular weight. Although used frequently, SDS-PAGE is a poor technique for quantitative protein purity due to inherent sample preparation artifacts, migration time a...

The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 1...

BACKGROUND Conventional immunoassays underestimate the urinary albumin concentration because intact albumin in urine exists in two forms, immunoreactive and immunochemically nonreactive. METHODS Urinary albumin concentration measured by HPLC (which measures total albumin, i.e., the sum of immunoreactive albumin + immunochemically nonreactive albumin) or RIA was compared with densitometric ana...

background: dicrocoelium dendriticum, a liver trematode of herbivores, and man is very prevalent among ruminants of iran, resulting in causing significant economic losses. recently its phenotype polymorphism has been found in iran, a phenomenon that may affect the protein profile of the parasite. objectives: the aim of this study was to determine the somatic protein profiles of d.dendriticum in...

We have purified and further characterized a histamine releasing factor (HRF) derived from human mononuclear cells using gel-filtration HPLC, reverse-phase HPLC, anion exchange chromatography, and elution from SDS gels after electrophoresis. Considerable heterogeneity is seen, far exceeding that published in prior reports. Gel filtration HPLC yielded a major peak at molecular weight 30,000 and ...