The multilayers of poly(L-lysine) (PLL) and hyaluronic acid (HA) were constructed by alternating deposition of PLL at high pH and HA at low pH. The exponential growth of the multilayer was proved to be amplified by increasing the pH difference between the two deposition solutions. The exponential growth multilayers of PLL/HA assembled at different pH were utilized as reservoirs for loading a tr...

Three crops of cucumber were grown in succession in beds by use of trickle irrigation, plastic film mulch, and soil chemical treatments over a 17-month period, including a fallow winter season. Total yield for the three crops was highest (1208 quintals/ha) in film-mulched plots treated with MBR-CP, and next-highest in film-mulched plots treated with DD-MENCS (1094 quintals/ha); total yield was ...

Thermally induced liquid-phase deposition method was employed to produce a thin hydroxyapatite (HA) film on a titanium substrate in a metastable calcium phosphate solution. Titanium foil of 100x10x0.02 mm3 was used as a substrate. Prior to HA coating, the substrate was immersed in 5 M NaOH solution at 60 degrees C for 24 hours. Substrate temperature was kept constant at 60 degrees C for 0.5-3 h...

Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time...

Hemoglobin (Hb) in the hyaluronic acid (HA) was cast at pyrolytic graphite (PG) electrodes for researching its electrochemical and electrocatalytic properties. The formal potential and electron transfer rate constant of Hb on HA films were determined, and the stability of the films, the pH effect, and the influence of supporting electrolyte concentrations upon Hb electrochemistry on the films w...

We present a quantitative comparison of the magnetization measured by spin-polarized neutron reflectivity (SPNR) and DC magnetometry on a 1370 Å -thick Nb superconducting film. As a function of magnetic field applied in the film plane, SPNR exhibits reversible behavior whereas the DC magnetization shows substantial hysteresis. The difference between these measurements is attributed to a rotatio...

PURPOSE This study was performed to separately assess the aqueous flow applied with hyaluronic acid, and the behaviour of hyaluronic acid itself on the ocular surface. METHODS Two different fluorescent dyes, fluorescein sodium dissolved in 0.1% hyaluronic acid (HA) solution and 0.1% fluorescein conjugated with hyaluronic acid (F-HA) dissolved in saline, were used. A volume of 20 mul of tested...

Materials Sodium form of hyaluronic acid (HA, MW, 10 kDa) was a gift of Kolon Life Science, Korea. EDC.HCl (99%) and N-Hydroxy succinimide (NHS, 99%) were purchased from Shanghai Medeep Co., Ltd. Monostearin (glycerol α-monostearate, GMS), Sodium deoxycholate (DOC) and fluorescein isothiocyanate (FITC) were purchased from Solarbio (Beijing, China). Doxorubicin hydrochloride (DOX) was supplied b...

Scanning- and colloidal-probe atomic force microscopy were used to study the mechanical properties of poly(L-lysine)/hyaluronan (PLL/HA)(n) films as a function of indentation velocity and the number of polymer deposition steps n. The film thickness was determined by two independent AFM-based methods: scratch-and-scan and newly developed full-indentation. The advantages and disadvantages of both...

A multilayered hydrogel film system based on hyaluronic acid-cysteamine (HA-Cym) and polyvinylalcohol (PVA) was fabricated. It contained a drug-impermeable backing layer, a supporting layer preventing direct contact between the loaded drug and the backing layer, a drug-loading layer and a mucoadhesive layer. Scanning electron microscopy demonstrated the presence of the distinct layers. The comp...