We have introduced a G-quadruplex-binding ligand, 3,6-bis(1-methyl-4-vinylpyridinium)carbazole diiodide (BMVC), to verify the major structure of d(T2AG3)4 (H24) in potassium solution and examine the structural conversion of H24 in sodium solution upon potassium titration. The studies of circular dichroism, induced circular dichroism, spectral titration and gel competition have allowed us to det...

General features of the manne lysogenic bacterium H24 are described. The bacterial strain was isolated from a sample of North Sea water in 1978 After several consecutive transfers on seawater agar slants spontaneous plaque-formation was observed in 1981 When H24 was grown in bouillon the cultures were found to contain plaque forming units (PFU) at any number between zero and 10%1-', indicating ...

BACKGROUND This study was design to investigate the prognostic value for death at day-28 of lactate course and lactate clearance during the first 24 hours in Intensive Care Unit (ICU), after initial resuscitation. METHODS Prospective, observational study in one surgical ICU in a university hospital. Ninety-four patients hospitalized in the ICU for severe sepsis or septic shock were included. ...

INTRODUCTION Because of disturbed renal autoregulation, patients experiencing hypotension-induced renal insult might need higher levels of mean arterial pressure (MAP) than the 65 mmHg recommended level in order to avoid the progression of acute kidney insufficiency (AKI). METHODS In 217 patients with sustained hypotension, enrolled and followed prospectively, we compared the evolution of the...

INTRODUCTION Urinary indices have limited effectiveness in separating transient acute kidney injury (AKI) from persistent AKI in ICU patients. Their time-course may vary with the mechanism of AKI. The primary objective of this study was to evaluate the diagnostic value of changes over time of the usual urinary indices in separating transient AKI from persistent AKI. METHODS An observational p...

There is considerable interest in understanding how the energy budget of an endotherm is modulated from a physiological and ecological point of view. In this paper, we used daily (24 h) heart rate (f(H24)), as a proxy of daily energy expenditure (DEE) across seasons, to test the effect of locomotion activity and water temperature on the energy budget of a large diving bird. f(H24) was monitored...

The marine lysogenic bacterium H24, known for the genetic instability of the resident wild-type phage $H24 when propagated in nutrient-rich medlum, was investigated by cultivation in synthet~c seawater enriched to vanous levels of yeast extract plus peptone (YEP, In a relation of 1:5). The incidence of phage mutants was found to depend on a balanced equilibrium of nutnent concentration (tested ...

Serum GM3 molecular species were quantified in 125 Japanese residents using tandem mass spectrometry multiple reaction monitoring. Individuals were categorized by the presence or absence of metabolic disease risk factors including visceral fat accumulation, hyperglycemia and dyslipidemia. A total of 23 GM3 molecular species were measured, of these, eight were found to be significantly elevated ...

Gluconobacter oxydans is characterized by its ability to incompletely oxidize carbohydrates and alcohols. The high yields of its oxidation products and complete secretion into the medium make it important for industrial use. We report the finished genome sequence of Gluconobacter oxydans H24, an industrial strain with high l-sorbose productivity.

Azotobacter vinelandii stimulated the growth of Agrobacterium tumefaciens H2, H23, H24, H27, and ATCC 15955 on media containing insoluble iron sources. The Azotobacter vinelandii siderophores appeared to promote Agrobacterium tumefaciens growth by solubilizing mineral iron, and the ferrisiderophores so formed then acted as iron sources for Agrobacterium tumefaciens. Agrobactin, the Agrobacteriu...