Objective (s): Titanium dioxide nanoparticles (TiO2 NPs) have been recognized as biologically inert material and have been used in a multitude of applications. Nevertheless, the negative impact on the human health is not yet well understood. Aim of the work: The study attempted to evaluate the epigenetic changes of the genome, in the form of DNA methylation in liver tissue samples, resulting fr...

s:1993:"Background: There are many approaches that gene expression is controlled in eukaryotes. DNA methylation is one of several epigenetic mechanisms that cells use to control gene expression and lock genes in the "off" position. In addition, sperm DNA damage can correlate with DNA methylation defect. There is evidence that sperm of infertile men contain more DNA damage than fertile men and t...

objective: to determine the role of global genome methylation in gastritis lesion and its relation with clinicopathologic finding. materials and methods: the study was conducted on 44 gastritis and normal adjacent specimens using a technique composed of restriction enzyme digestion and pyrosequencing known as luma (luminometric methylation assay). at first, dna extracted from gastritis lesion a...

methylated and un-methylated control dna is an important part of dna methylation studies. although human and mouse dna methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. in this paper a simple method of generating methylated and un-methylated control dna is described. whole...

Methylated and un-methylated control DNA is an important part of DNA methylation studies. Although human and mouse DNA methylation control sets are commercially available, in case of methylation studies on other species such as animals, plants, and bacteria, control sets need to be prepared. In this paper a simple method of generating methylated and un-methylated control DNA is described. Whole...

Down syndrome (DS, also known as trisomy 21) most often results from chromosomal nondisjunction during oogenesis. Numerous studies sustain a causal link between global DNA hypomethylation and genetic instability. It has been suggested that DNA hypomethylation might affect the structure and dynamics of chromatin regions that are critical for chromosome stability and segregation, thus favouring c...

for a long time, scientists have tried to describe disorders just by genetic or environmental factors. however, the role of epigenetics in human diseases has been considered from a half of century ago. in the last decade, this subject has attracted many interests, especially in complicated disorders such as behavior plasticity, memory, cancer, autoimmune disease, and addiction as well as neurod...