نتایج جستجو برای: Gliadin

تعداد نتایج: 1324  

Journal: :Bioscience, biotechnology, and biochemistry 2007
Hitomi Kumagai Akihiro Suda Hidetoshi Sakurai Hitoshi Kumagai Soichi Arai Naoko Inomata Zenro Ikezawa

Wheat gliadin was deamidated by using a cation-exchange resin in the presence or absence of added cysteine, with the change in digestibility being measured. The allergenicity of the gliadin was evaluated by using sera from patients RAST-positive to wheat. Gliadin-specific IgE was measured after the gliadin had been orally administered to rats. The addition of cysteine before the treatment with ...

Journal: :Gut 2000
E H Arentz-Hansen S N McAdam O Molberg C Kristiansen L M Sollid

BACKGROUND/AIMS Coeliac disease is a chronic intestinal disorder most probably caused by an abnormal immune reaction to wheat gliadin. The identification of the HLA-DQ2 and HLA-DQ8 as the molecules responsible for the HLA association in coeliac disease strongly implicates a role for CD4 T cells in disease pathogenesis. Indeed, CD4 T cells specific for gliadin have been isolated from the small i...

2016
Sung-Hoon Moon Jihun Kim Mi-Young Kim Do Hyun Park Tae Jun Song Sun A Kim Sang Soo Lee Dong Wan Seo Sung Koo Lee Myung-Hwan Kim

BACKGROUND/AIMS The aim of this study was to establish a pathogenetic mechanism of pancreatitis in celiac disease and IgG4-related disease using gluten-sensitive human leukocyte antigen (HLA)-DQ8 transgenic mice. METHODS Transgenic mice expressing HLA-DQ8 genes were utilized. Control mice were not sensitized but were fed gliadin-free rice cereal. Experimental groups consisted of gliadin-sensi...

Journal: :Annales de biologie clinique 2010
Laurence Lutteri Clémence Sagot Jean-Paul Chapelle

Recently, anti-deamidated gliadin antibodies were proposed for the serological diagnosis of celiac disease. We evaluate the specificity of different anti-deamidated gliadin antibodies ELISA in comparison with conventional anti-native gliadin kits. Serum samples from 46 non celiac patients were analyzed by five different quantitative ELISA for anti-native gliadin, anti-deamidated gliadin and ant...

Journal: :Nutritional neuroscience 2004
A Vojdani T O'Bryan J A Green J Mccandless K N Woeller E Vojdani A A Nourian E L Cooper

The mechanisms behind autoimmune reaction to nervous system antigens in autism are not understood. We assessed the reactivity of sera from 50 autism patients and 50 healthy controls to specific peptides from gliadin and the cerebellum. A significant percentage of autism patients showed elevations in antibodies against gliadin and cerebellar peptides simultaneously. For examining cross-reaction ...

امیری, رضا , دخانی, شهرام , رضایی, عبدالمجید , شاهدی, محمد,

This study was conducted to evaluate the capability of reversed-phase high performance liquid chromatography (RP-HPLC) of wheat (Triticum aestivum L.) storage proteins, and their genetic variability in different winter and spring isolines, cultivars and landraces. Gliadin proteins were extracted from the flour of five randomly selected seeds of each genotype. In this method, Nucleosil C18 300 A...

2013
Merlin Nanayakkara Giuliana Lania Mariantonia Maglio Roberta Kosova Marco Sarno Alessandra Gaito Valentina Discepolo Riccardo Troncone Salvatore Auricchio Renata Auricchio Maria Vittoria Barone

Celiac disease (CD) occurs frequently, and is caused by ingestion of prolamins from cereals in subjects with a genetic predisposition. The small intestinal damage depends on an intestinal stress/innate immune response to certain gliadin peptides (e.g., A-gliadin P31-43) in association with an adaptive immune response to other gliadin peptides (e.g., A-gliadin P57-68). Gliadin and peptide P31-43...

Journal: :Biopolymers 2009
Li Liang Maud Pinier Jean-Christophe Leroux Muriel Subirade

The wheat gluten protein alpha-gliadin, a well known trigger of celiac disease, can be complexed by random copolymers of hydroxyethyl methacrylate (HEMA) and sodium 4-styrene sulfonate (SS). In this work, influence of alpha-gliadin and poly(HEMA-co-SS) concentrations on alpha-gliadin structure was studied using spectroscopic techniques and dynamic light scattering. In 70% ethanol or 0.06M HCl (...

Journal: :Journal of immunology 2007
Giuseppe Terrazzano Michela Sica Carmen Gianfrani Giuseppe Mazzarella Francesco Maurano Beatrice De Giulio Sophie de Saint-Mezard Delia Zanzi Luigi Maiuri Marco Londei Bana Jabri Riccardo Troncone Salvatore Auricchio Serafino Zappacosta Ennio Carbone

We analyzed the autologous NK cell interaction with gliadin-presenting dendritic cells. Gliadin is the known Ag priming the celiac disease (CD) pathogenesis. We demonstrate that gliadin prevents immature dendritic cells (iDCs) elimination by NK cells. Furthermore, cooperation between human NK cells-iDCs and T cells increases IFN-gamma production of anti-gliadin immune response. Gliadin fraction...

2013
Narendra Reddy Yiqi Yang

For the first time, gliadin films with excellent strength and water stability have been developed without using any crosslinking agents. So far, it has not been possible to obtain water stable gliadin films even after crosslinking. In this research, a novel method of using aqueous urea and ethanol has been developed to obtain highly water stable gliadin films without using crosslinking chemical...

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