background mycobacterium tuberculosis genotyping can effectively improve tuberculosis (tb) control programs by controlling disease transmission. pulsed field gel electrophoresis (pfge) is a particularly powerful tool for determination of clonal identity of bacteria providing information for understanding and controlling the spread of disease. objectives the aim of present study was to investiga...

background: retinoblastoma is the most common intraocular tumor in childhood and mutation in the rb1 gene will trigger the tumorigenesis. so far, a wide range of the mutations along the length of rb1 gene have been reported. however, some could not be detected by common detection methods. in such condition, linkage analysis using microsatellite markers is suggested to trace unknown rb1 mutation...

conclusions in our study, only hbv genotype d was found among all the positive hbsag and occult hbv infection. moreover, high prevalence (40.7%) of occult hbv infection was determined among patients suffered from cryptogenic cirrhosis. results eleven (40.7%) out of 27 hbv cryptogenic cirrhosis and all 11 hbsag-positive patients were positive for hbv dna. the seroprevalences of hepatitis b virus...

background: classification of molar gestation into complete hydatidiform mole (chm) and partial hydatidiform mole (phm) is done according to clinical, ultrasonographic, histologic and genetic criteria. however, making a distinction between chm and phm using histologic criteria alone may be difficult and several studies have shown that misclassifications are frequent, even for experienced pathol...

objectives: this current research was conducted in order to analyze the molecular characterization of isolated cryptosporidium spp. in the southwest of iran. materials and methods: in this survey, 390 fecal samples were collected from immunocompromised individuals and children under five-years-of-age. parasitic infection was evaluated using wet mount preparation, formalin ether, a modified acid...

methods and materials: hepatitis b virus dna was detected by conventional pcr in some of the serum samples obtained from hbsag-positive chronic hepatitis b (chb) patients who were referred to health centers of shahrekord for routine monitoring of the disease. subsequently, using real-time pcr, the dna samples were used for genotyping and analysis of resistance to lamivudine. results: the dna wa...

background: group b streptococcus (gbs) is the major cause of serious life threatening infections in neonates, pregnant women, and other adults with underlying diseases. capsular polysaccharide typing is a significant way for epidemiological studies of gbs, the pathogenesis, and other studies associated with gbs infections including surveillance programs and vaccine development in future. molec...

abstract        background and objective: paratuberculosis is a chronic granulomatous enteritis of ruminants caused by mycobacterium avium subspecies paratuberculosis (map). this study aimed to characterize the genome of the map 316f strain.       methods: the map 316f strain was subjected to the pcr-f57 and pcr-is900 experiments in order to ensure its identity as map. this was followed by appl...

conclusions findings of the present study showed that hcv genotype 3 was the predominant genotype followed by the subtypes 1a and 1b in yazd, central province of iran. in addition, there was no difference between hcv load and genotypes 1 and 3. hcv genotyping is recommended in other provinces of iran. objectives the main aim of this study was to estimate the prevalence of hcv genotypes in yazd,...

conclusions the determination of serotype and surface proteins of gbs strains distribution would ‎be ‎relevant ‎for the future possible formulation of a gbs vaccine. patients and methods in this cross-sectional study, a total of 100 gbs strains isolated from 237 pregnant and 413 non-pregnant women were investigated for molecular capsular serotypes and surface protein genes using the multiplex p...